Superoxide Dismutase 1 (SOD1)-Derived Peptide Inhibits Amyloid Aggregation of Familial Amyotrophic Lateral Sclerosis SOD1 Mutants

Victor Banerjee, Tom Shani, Bella Katzman, Maria Vyazmensky, Niv Papo, Adrian Israelson, Stanislav Engel

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder that leads to the death of the upper and lower motor neurons. Superoxide dismutase 1 (SOD1) is an ALS pathogenic protein, whose misfolding results in the formation of amyloid aggregates. The mechanism underlying SOD1 pathogenesis in ALS remains obscure, but one possible mechanism involves gain-of-interaction, in which the misfolded soluble SOD1 forms abnormal protein-protein interactions (PPIs) with various cellular proteins, including with other SOD1 molecules, thereby interfering with their function. The structural basis of this gain-of-interaction mechanism is unknown. Here, we characterized the backbone dynamics landscape of misfolded SOD1 to pinpoint surface areas predisposed to aberrant PPIs. This analysis enabled us to formulate a working hypothesis for the mechanism of the gain-of-function of misfolded SOD1, according to which an abnormal PPI potential results from the increased mobility of the SOD1 surface backbone. Guided by the backbone dynamics landscape, we have identified a SOD1-derived peptide that can bind SOD1 proteins and divert the typical amyloid aggregation of ALS-related SOD1 mutants toward a potentially less toxic amorphous aggregation pathway.

Original languageEnglish
Pages (from-to)1595-1606
Number of pages12
JournalACS Chemical Neuroscience
Volume7
Issue number11
DOIs
StatePublished - 16 Nov 2016

Keywords

  • Amyotrophic lateral sclerosis (ALS)
  • amyloid aggregation
  • gain-of-function
  • stability patches
  • steered molecular dynamics (SMD)
  • superoxide dismutase 1 (SOD1)

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