Superoxide generated from the glutathione-mediated reduction of selenite damages the iron-sulfur cluster of chloroplastic ferredoxin

Brian Fisher, Dmitry Yarmolinsky, Salah Abdel-Ghany, Marinus Pilon, Elizabeth A. Pilon-Smits, Moshe Sagi, Doug Van Hoewyk

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Selenium assimilation in plants is facilitated by several enzymes that participate in the transport and assimilation of sulfate. Manipulation of genes that function in sulfur metabolism dramatically affects selenium toxicity and accumulation. However, it has been proposed that selenite is not reduced by sulfite reductase. Instead, selenite can be non-enzymatically reduced by glutathione, generating selenodiglutathione and superoxide. The damaging effects of superoxide on iron-sulfur clusters in cytosolic and mitochondrial proteins are well known. However, it is unknown if superoxide damages chloroplastic iron-sulfur proteins. The goals of this study were twofold: to determine whether decreased activity of sulfite reductase impacts selenium tolerance in Arabidopsis, and to determine if superoxide generated from the glutathione-mediated reduction of selenite damages the iron-sulfur cluster of ferredoxin. Our data demonstrate that knockdown of sulfite reductase in Arabidopsis does not affect selenite tolerance or selenium accumulation. Additionally, we provide in vitro evidence that the non-enzymatic reduction of selenite damages the iron-sulfur cluster of ferredoxin, a plastidial protein that is an essential component of the photosynthetic light reactions. Damage to ferredoxin's iron-sulfur cluster was associated with formation of apo-ferredoxin and impaired activity. We conclude that if superoxide damages iron-sulfur clusters of ferredoxin in planta, then it might contribute to photosynthetic impairment often associated with abiotic stress, including toxic levels of selenium.

Original languageEnglish
Pages (from-to)228-235
Number of pages8
JournalPlant Physiology and Biochemistry
Volume106
DOIs
StatePublished - 1 Sep 2016

Keywords

  • Ferredoxin
  • Iron-sulfur cluster
  • Oxidative stress
  • Selenium
  • Superoxide

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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