Surface-Enhanced Raman Spectroscopy as a Tool for Probing Specific Biochemical Components in Bacteria

L. Zeiri; B. V. Bronk; Y. Shabtai; J. Eichler; S. Efrima

doi:10.1366/000370204322729441

Surface-Enhanced Raman Spectroscopy as a Tool for Probing Specific Biochemical Components in Bacteria

L. Zeiri, B. V. Bronk, Y. Shabtai, J. Eichler, S. Efrima

Research output: Contribution to journal › Article › peer-review

211 Scopus citations

Abstract

Treatment of bacteria with silver yields intense and highly specific surface-enhanced Raman spectroscopy (SERS) spectra from various cellular chemical components located in the vicinity of the silver colloids. In particular, we demonstrate an extreme sensitivity to flavin components associated with the cell envelope and to their state of oxidation. Different spectra, possibly associated with DNA, carboxylates, and perhaps phosphates, are obtained from the soluble interior fraction of the cell.

Original language	English
Pages (from-to)	33-40
Number of pages	8
Journal	Applied Spectroscopy
Volume	58
Issue number	1
DOIs	https://doi.org/10.1366/000370204322729441
State	Published - 1 Jan 2004

Keywords

Bacteria
Flavins
Silver colloids
Surface-enhanced Raman scattering

ASJC Scopus subject areas

Instrumentation
Spectroscopy

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10.1366/000370204322729441

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abstract = "Treatment of bacteria with silver yields intense and highly specific surface-enhanced Raman spectroscopy (SERS) spectra from various cellular chemical components located in the vicinity of the silver colloids. In particular, we demonstrate an extreme sensitivity to flavin components associated with the cell envelope and to their state of oxidation. Different spectra, possibly associated with DNA, carboxylates, and perhaps phosphates, are obtained from the soluble interior fraction of the cell.",

keywords = "Bacteria, Flavins, Silver colloids, Surface-enhanced Raman scattering",

author = "L. Zeiri and Bronk, {B. V.} and Y. Shabtai and J. Eichler and S. Efrima",

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AU - Zeiri, L.

AU - Bronk, B. V.

AU - Shabtai, Y.

AU - Eichler, J.

AU - Efrima, S.

PY - 2004/1/1

Y1 - 2004/1/1

N2 - Treatment of bacteria with silver yields intense and highly specific surface-enhanced Raman spectroscopy (SERS) spectra from various cellular chemical components located in the vicinity of the silver colloids. In particular, we demonstrate an extreme sensitivity to flavin components associated with the cell envelope and to their state of oxidation. Different spectra, possibly associated with DNA, carboxylates, and perhaps phosphates, are obtained from the soluble interior fraction of the cell.

AB - Treatment of bacteria with silver yields intense and highly specific surface-enhanced Raman spectroscopy (SERS) spectra from various cellular chemical components located in the vicinity of the silver colloids. In particular, we demonstrate an extreme sensitivity to flavin components associated with the cell envelope and to their state of oxidation. Different spectra, possibly associated with DNA, carboxylates, and perhaps phosphates, are obtained from the soluble interior fraction of the cell.

KW - Bacteria

KW - Flavins

KW - Silver colloids

KW - Surface-enhanced Raman scattering

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U2 - 10.1366/000370204322729441

DO - 10.1366/000370204322729441

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ER -

Surface-Enhanced Raman Spectroscopy as a Tool for Probing Specific Biochemical Components in Bacteria

Abstract

Keywords

ASJC Scopus subject areas

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