Surface-Enhanced Raman Spectroscopy as a Tool for Probing Specific Biochemical Components in Bacteria

L. Zeiri, B. V. Bronk, Y. Shabtai, J. Eichler, S. Efrima

Research output: Contribution to journalArticlepeer-review

226 Scopus citations

Abstract

Treatment of bacteria with silver yields intense and highly specific surface-enhanced Raman spectroscopy (SERS) spectra from various cellular chemical components located in the vicinity of the silver colloids. In particular, we demonstrate an extreme sensitivity to flavin components associated with the cell envelope and to their state of oxidation. Different spectra, possibly associated with DNA, carboxylates, and perhaps phosphates, are obtained from the soluble interior fraction of the cell.

Original languageEnglish
Pages (from-to)33-40
Number of pages8
JournalApplied Spectroscopy
Volume58
Issue number1
DOIs
StatePublished - 1 Jan 2004

Keywords

  • Bacteria
  • Flavins
  • Silver colloids
  • Surface-enhanced Raman scattering

ASJC Scopus subject areas

  • Instrumentation
  • Spectroscopy

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