Survival of introduced Tuber melanosyorum at two sites in Israel as measured by occurrence of mycorrhizas

V. Kagan-Zur, S. Freeman, Y. Luzzati, Nurit Bejerano, E. Shabi

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    4 Scopus citations

    Abstract

    In the framework of efforts to introduce Tuber melanosporum as a cultivated crop to Israel, spores of the truffle, obtained from fruit-bodies procured in Italy and France, were used to inoculate oak seedlings and hazel suckers. Typical T. melanosporum mycorrhizas were observed 3 months after inoculation on roots of both plant species. One- to two-year-old mycorrhizal seedlings were outplanted at a number of experimental sites and irrigated regularly. Two sites characterized by alkaline soil but differing in soil composition and climatic conditions were chosen for the present study. DNA of ascocarps used for inoculation, DNA of re-isolated cultures and fungal DNA taken from tree mycorrhizas 4 years after outplanting were compared with T. melanosporum reference cultures by molecular methods. All T. melanosporum profiles proved to be identical except for one belonging to a reference culture, which exhibited an unusual HinfI ITS-RFLP pattern. A single base substitution, responsible for the different HinfI restriction site, distinguished the ITS region of this culture from a published T. melanosporum ITS sequence. ITS restriction polymorphism analyses determined that roots of all potted plants tested and many 4-year-old trees from the two experimental plots (irrespective of soil and climatic differences) were colonized by T. melanosporum.

    Original languageEnglish
    Pages (from-to)159-166
    Number of pages8
    JournalPlant and Soil
    Volume229
    Issue number2
    DOIs
    StatePublished - 22 Mar 2001

    Keywords

    • ITS-RFLP
    • Mycorrhiza survival
    • Tuber melanosporum

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