TY - JOUR
T1 - Synaptic release of zinc from brain slices
T2 - Factors governing release, imaging, and accurate calculation of concentration
AU - Frederickson, Christopher J.
AU - Giblin, Leonard J.
AU - Rengarajan, Balaji
AU - Masalha, Rafik
AU - Frederickson, Cathleen J.
AU - Zeng, Yaping
AU - Lopez, Emilio Varea
AU - Koh, Jae Young
AU - Chorin, Udi
AU - Besser, Limor
AU - Hershfinkel, Michal
AU - Li, Yang
AU - Thompson, Richard B.
AU - Krezel, Artur
PY - 2006/6/30
Y1 - 2006/6/30
N2 - Cerebrocortical neurons that store and release zinc synaptically are widely recognized as critical in maintenance of cortical excitability and in certain forms of brain injury and disease. Through the last 20 years, this synaptic release has been observed directly or indirectly and reported in more than a score of publications from over a dozen laboratories in eight countries. However, the concentration of zinc released synaptically has not been established with final certainty. In the present work we have considered six aspects of the methods for studying release that can affect the magnitude of zinc release, the imaging of the release, and the calculated concentration of released zinc. We present original data on four of the issues and review published data on two others. We show that common errors can cause up to a 3000-fold underestimation of the concentration of released zinc. The results should help bring consistency to the study of synaptic release of zinc.
AB - Cerebrocortical neurons that store and release zinc synaptically are widely recognized as critical in maintenance of cortical excitability and in certain forms of brain injury and disease. Through the last 20 years, this synaptic release has been observed directly or indirectly and reported in more than a score of publications from over a dozen laboratories in eight countries. However, the concentration of zinc released synaptically has not been established with final certainty. In the present work we have considered six aspects of the methods for studying release that can affect the magnitude of zinc release, the imaging of the release, and the calculated concentration of released zinc. We present original data on four of the issues and review published data on two others. We show that common errors can cause up to a 3000-fold underestimation of the concentration of released zinc. The results should help bring consistency to the study of synaptic release of zinc.
KW - Cerebrocortical neurons
KW - Synaptic release
KW - Zinc
UR - http://www.scopus.com/inward/record.url?scp=33744545500&partnerID=8YFLogxK
U2 - 10.1016/j.jneumeth.2005.11.014
DO - 10.1016/j.jneumeth.2005.11.014
M3 - Article
AN - SCOPUS:33744545500
SN - 0165-0270
VL - 154
SP - 19
EP - 29
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
IS - 1-2
ER -