TY - JOUR
T1 - Synergistic effect of interferon-γ and phorbol myristate acetate on superoxide production by human monocytes.
AU - Wolfson, Marina
AU - Nathan, Ilana
AU - Aharon, Miriam
AU - Dvilansky, Alexander
AU - Aboud, Mordechai
PY - 1993/6/1
Y1 - 1993/6/1
N2 - This study demonstrates a synergistic effect of IFNγ and PMA on superoxide generation by human monocytes. A strict correlation was observed between the induction of superoxide production and PK-C activation by PMA alone. No such correlation was evident for IFNγ. However, exposure of the cells to IFNγ for 10 to 15 hr prior to PMA treatment enhanced both superoxide production and PK-C activation. Using protein kinase inhibitors, we noticed that while PMA exerted its effect by activating PK-C, IFNγ operated via activation of calcium/calmodulin-dependent or some other calcium-dependent protein kinases. These kinases appeared to be involved in the effect of IFNγ on superoxide production, as well as in its potentiation of PMA activity.
AB - This study demonstrates a synergistic effect of IFNγ and PMA on superoxide generation by human monocytes. A strict correlation was observed between the induction of superoxide production and PK-C activation by PMA alone. No such correlation was evident for IFNγ. However, exposure of the cells to IFNγ for 10 to 15 hr prior to PMA treatment enhanced both superoxide production and PK-C activation. Using protein kinase inhibitors, we noticed that while PMA exerted its effect by activating PK-C, IFNγ operated via activation of calcium/calmodulin-dependent or some other calcium-dependent protein kinases. These kinases appeared to be involved in the effect of IFNγ on superoxide production, as well as in its potentiation of PMA activity.
UR - http://www.scopus.com/inward/record.url?scp=0027158823&partnerID=8YFLogxK
U2 - 10.1006/cbir.1993.1104
DO - 10.1006/cbir.1993.1104
M3 - Article
AN - SCOPUS:0027158823
SN - 1065-6995
VL - 17
SP - 595
EP - 602
JO - Cell Biology International
JF - Cell Biology International
IS - 6
ER -