Synthesis and characterization of a pyrrole-alginate conjugate and its application in a biosensor construction

Khalil Abu-Rabeah, Boris Polyak, Rodica E. Ionescu, Serge Cosnier, Robert S. Marks

Research output: Contribution to journalArticlepeer-review

84 Scopus citations

Abstract

N-(3-Aminopropyl)pyrrole was covalently coupled with alginate in an aqueous-phase reaction by means of carbodiimide-mediated activation chemistry to provide a pyrrole-alginate conjugate for subsequent use in biosensor applications. The pyrrole-alginate conjugate was quantified by UV spectroscopy at 230 nm, by an HPSEC-MALLS analytical method, as well as by FTIR and 13C NMR spectroscopies. The new pyrrole-alginate conjugate was used for the immobilization of polyphenol oxidase (PPO) onto an electrode surface by physical entrapment resulting from the gellification process and electrochemical polymerization of the pyrrole groups. The efficiency of this cross-linking approach (chemical and electrochemical) was investigated by comparing the amount of enzyme released from polypyrrole-alginate and regular alginate. In addition, biosensors were prepared by entrapment of the PPO in polypyrrole-alginate and regular alginate matrixes and their performance for the amperometric determination of catechol chosen as a model analyte was examined, yielding a sensitivity of 350 and 80,μA M-1 cm-2, respectively, for polypyrrole-alginate and alginate biosensors.

Original languageEnglish
Pages (from-to)3315-3318
Number of pages4
JournalBiomacromolecules
Volume6
Issue number6
DOIs
StatePublished - 1 Nov 2005

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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