TY - JOUR
T1 - Synthesis and characterization of an electroactive surface that releases γ-aminobutyric acid (GABA)
AU - Yan, Chun
AU - Matsuda, Wakana
AU - Pepperberg, David R.
AU - Zimmerman, Steven C.
AU - Leckband, Deborah E.
N1 - Funding Information:
This work was supported by the University of Illinois Intercampus Research Initiative in Biotechnology (IRIB) program and by grant EY13693 from the National Institutes of Health. C. Yan was supported in part by DOE award number DEFGO2-91ER45439. The XPS facilities used are in the Center for Microanalysis of Materials, University of Illinois at Urbana-Champaign, which is partially supported by the U.S. Department of Energy under Grant DEFG02-91-ER45439. D.R.P. is a Senior Scientific Investigator of Research to Prevent Blindness (New York, NY).
PY - 2006/4/1
Y1 - 2006/4/1
N2 - We report the synthesis and characterization of a new electroactive surface capable of releasing the neurotransmitter γ-aminobutyric acid (GABA) upon reduction. The GABA was anchored to an alkanethiol via electrochemically active quinone (abbreviation, TM-GABA). The quinone unit, upon reduction to the hydroquinone, cyclizes to release GABA into solution. The half-life is 99 s. The self-assembled monolayer (SAM) of TM-GABA on gold was prepared and characterized with several surface sensitive techniques. X-ray photoelectron spectroscopy (XPS) explored the SAM formation of TM-GABA on Au surfaces. Cyclic voltammograms showed the ability to electrochemically control the quinone unit at the distal end of the chain. GABA was selectively released upon electrochemical reduction at a potential of -700 mV. The functional GABA terminal group was detected by surface plasmon resonance measurements of anti-GABA antibody binding.
AB - We report the synthesis and characterization of a new electroactive surface capable of releasing the neurotransmitter γ-aminobutyric acid (GABA) upon reduction. The GABA was anchored to an alkanethiol via electrochemically active quinone (abbreviation, TM-GABA). The quinone unit, upon reduction to the hydroquinone, cyclizes to release GABA into solution. The half-life is 99 s. The self-assembled monolayer (SAM) of TM-GABA on gold was prepared and characterized with several surface sensitive techniques. X-ray photoelectron spectroscopy (XPS) explored the SAM formation of TM-GABA on Au surfaces. Cyclic voltammograms showed the ability to electrochemically control the quinone unit at the distal end of the chain. GABA was selectively released upon electrochemical reduction at a potential of -700 mV. The functional GABA terminal group was detected by surface plasmon resonance measurements of anti-GABA antibody binding.
KW - Cyclic voltammogram
KW - Electrochemical reduction
KW - GABA
KW - SPR
KW - Self-assembled monolayers (SAMs)
KW - XPS
UR - http://www.scopus.com/inward/record.url?scp=33644530700&partnerID=8YFLogxK
U2 - 10.1016/j.jcis.2005.08.029
DO - 10.1016/j.jcis.2005.08.029
M3 - Article
C2 - 16168426
AN - SCOPUS:33644530700
SN - 0021-9797
VL - 296
SP - 165
EP - 177
JO - Journal of Colloid and Interface Science
JF - Journal of Colloid and Interface Science
IS - 1
ER -