Synthesis and delivery of a stable phosphorylated ubiquitin probe to study ubiquitin conjugation in mitophagy

Guy Mann; Gandhesiri Satish; Prasad Sulkshane; Shaswati Mandal; Michael H. Glickman; Ashraf Brik

doi:10.1039/d1cc04045f

Synthesis and delivery of a stable phosphorylated ubiquitin probe to study ubiquitin conjugation in mitophagy

Guy Mann, Gandhesiri Satish, Prasad Sulkshane, Shaswati Mandal, Michael H. Glickman, Ashraf Brik

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Protein post-translational modifications are involved in essentially all aspects of cellular signaling. Their dynamic nature and the difficulties in installing them using enzymatic approaches limits their direct study in human cells. Reported herein is the first synthesis, delivery and cellular study of a stable phosphoubiquitin probe. Our results compare Parkin's substrate preference during mitophagyviadirect visualization of a phosphorylated ubiquitin probe in the cellular environment.

Original language	English
Pages (from-to)	9438-9441
Number of pages	4
Journal	Chemical Communications
Volume	57
Issue number	74
DOIs	https://doi.org/10.1039/d1cc04045f
State	Published - 21 Sep 2021
Externally published	Yes

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/d1cc04045f

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abstract = "Protein post-translational modifications are involved in essentially all aspects of cellular signaling. Their dynamic nature and the difficulties in installing them using enzymatic approaches limits their direct study in human cells. Reported herein is the first synthesis, delivery and cellular study of a stable phosphoubiquitin probe. Our results compare Parkin's substrate preference during mitophagyviadirect visualization of a phosphorylated ubiquitin probe in the cellular environment.",

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AU - Sulkshane, Prasad

AU - Mandal, Shaswati

AU - Glickman, Michael H.

AU - Brik, Ashraf

PY - 2021/9/21

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AB - Protein post-translational modifications are involved in essentially all aspects of cellular signaling. Their dynamic nature and the difficulties in installing them using enzymatic approaches limits their direct study in human cells. Reported herein is the first synthesis, delivery and cellular study of a stable phosphoubiquitin probe. Our results compare Parkin's substrate preference during mitophagyviadirect visualization of a phosphorylated ubiquitin probe in the cellular environment.

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M3 - Article

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Synthesis and delivery of a stable phosphorylated ubiquitin probe to study ubiquitin conjugation in mitophagy

Abstract

ASJC Scopus subject areas

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