Synthesis of Leishmania cap-4 intermediates, cap-2 and cap-3

Magdalena Lewdorowicz, Janusz Stepinski, Ryszard Kierzek, Jacek Jemielity, Joanna Zuberek, Yael Yoffe, Michal Shapira, Ryszard Stolarski, Edward Darzynkiewicz

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Synthesis of Leishmania mRNA 5′-cap analogs, m7Gpppm26AmpAm (cap-2), and m7Gpppm26AmpAmpCm (cap-3) is reported. Binding affinities of those cap analogs for LeishIF4E proteins were determined using fluorescence spectroscopy. Cap-3 showed similar affinity to LeishIF4Es compared to the mature trypanosomatids cap structure (cap-4).

Original languageEnglish
Pages (from-to)1339-1348
Number of pages10
JournalNucleosides, Nucleotides and Nucleic Acids
Volume26
Issue number10-12
DOIs
StatePublished - 1 Oct 2007

Keywords

  • Cap analogs
  • Leishmania
  • Trypanosomatids

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Genetics

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