Synthesis of N-Hydroxy Isopeptide Containing Proteins

Suman Kumar Maity, Shimrit Ohayon, Ashraf Brik

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Chemical synthesis of a peptide-ubiquitin conjugate linked by an N-hydroxy isopeptide bond to determine what effect the N-hydroxy group has on the enzymatic hydrolysis of the isopeptide linkage by deubiquitinases is reported. This conjugate was subjected to proteolysis by UCH-L3 in the presence and absence of various metal ions, and no substantive difference in hydrolysis was seen compared to a control lacking the N-hydroxy group. The accessibility of N-hydroxy ubiquitinated substrates may find uses to study other deubiquitinases in particular those which use a zinc ion as a part of their catalytic mechanism.

Original languageEnglish
Pages (from-to)3438-3441
Number of pages4
JournalOrganic Letters
Issue number14
StatePublished - 17 Jul 2015
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry


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