Abstract
Induction of proliferation of human peripheral blood lymphocyte by T cell mitogens was found to be associated with dramatic increase in topoisomerase I DNA relaxing activity in the nuclear, but not in the cytosol, fraction. In addition, cell proliferation was associated with increased DNA topoisomerase I protein levels in both nuclear and cytosol fractions. Inhibitors of T cell proliferation abrogated both topoisomerase I protein levels and enzymatic activity. The results support a model in which cell proliferation is followed by increased DNA topoisomerase I protein levels and translocation of the enzymatically inactive molecule to the nucleus where additional regulatory mechanisms convert it into an active enzyme.
Original language | English |
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Pages (from-to) | 872-877 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 211 |
Issue number | 3 |
DOIs | |
State | Published - 26 Jun 1995 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology