T cell mitogens induce increased DNA topoisomerase I protein levels and its translocation to the nucleus where activation of the enzyme occurs

Sigal Gelkop, Esther Priel, Noah Isakov

Research output: Contribution to journalArticlepeer-review

Abstract

Induction of proliferation of human peripheral blood lymphocyte by T cell mitogens was found to be associated with dramatic increase in topoisomerase I DNA relaxing activity in the nuclear, but not in the cytosol, fraction. In addition, cell proliferation was associated with increased DNA topoisomerase I protein levels in both nuclear and cytosol fractions. Inhibitors of T cell proliferation abrogated both topoisomerase I protein levels and enzymatic activity. The results support a model in which cell proliferation is followed by increased DNA topoisomerase I protein levels and translocation of the enzymatically inactive molecule to the nucleus where additional regulatory mechanisms convert it into an active enzyme.

Original languageEnglish
Pages (from-to)872-877
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume211
Issue number3
DOIs
StatePublished - 26 Jun 1995

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