@article{63cc7ccd09c1488d8d72b4798c11c740,
title = "Talin-activated vinculin interacts with branched actin networks to initiate bundles",
abstract = "Vinculin plays a fundamental role in integrin-mediated cell adhesion. Activated by talin, it interacts with diverse adhesome components, enabling mechanical coupling between the actin cytoskeleton and the extracellular matrix. Here we studied the interactions of activated full-length vinculin with actin and the way it regulates the organization and dynamics of the Arp2/3 complex-mediated branched actin network. Through a combination of surface patterning and light microscopy experiments we show that vinculin can bundle dendritic actin networks through rapid binding and filament crosslinking. We show that vinculin promotes stable but flexible actin bundles having a mixed-polarity organization, as confirmed by cryo-electron tomography. Adhesion-like synthetic design of vinculin activation by surface-bound talin revealed that clustered vinculin can initiate and immobilize bundles from mobile Arp2/3-branched networks. Our results provide a molecular basis for coordinate actin bundle formation at nascent adhesions.",
author = "Rajaa Boujemaa-Paterski and Bruno Martins and Matthias Eibauer and Beales, {Charlie T.} and Benjamin Geiger and Ohad Medalia",
note = "Funding Information: This study was supported by the Swiss National Foundation, grant no. 31003A_179418, and the M{\"a}xi Foundation (to OM), the Israel Science Foundation (to BG) and the Klaus Tschira Foundation (to BG). The authors would like to thank the Center of Microscopy and Image Analysis of the University of Z{\"u}rich, and Sascha Weidner from the Workshop of the Department of Biochemistry, the University of Z{\"u}rich, for the design and production of the mask holder used for the micropatterning. The authors are grateful to Prof. Hans-Werner Fink, Prof. J{\"u}rg Osterwalder and Prof. Marta Gibert from the Physik-Institut of the University of Z{\"u}rich for their help with the UVO cleaner. We thank Ed Egelman (University of Virginia) for fruitful discussions during the initial stages of the project. We thank Julien Berro (Yale University) for insightful discussions. BG is the incumbent of the Erwin Neter Professorial Chair in Cell and Tumor Biology. We thank Dr Agnieszka Kawska (IllusScientia) for the artwork in Figure 5. Funding Information: This study was supported by the Swiss National Foundation, grant no. 31003A_179418, and the M?xi Foundation (to OM), the Israel Science Foundation (to BG) and the Klaus Tschira Foundation (to BG). The authors would like to thank the Center of Microscopy and Image Analysis of the University of Z?rich, and Sascha Weidner from the Workshop of the Department of Biochemistry, the University of Z?rich, for the design and production of the mask holder used for the micropatterning. The authors are grateful to Prof. Hans-Werner Fink, Prof. J?rg Osterwalder and Prof. Marta Gibert from the Physik-Institut of the University of Z?rich for their help with the UVO cleaner. We thank Ed Egelman (University of Virginia) for fruitful discussions during the initial stages of the project. We thank Julien Berro (Yale University) for insightful discussions. BG is the incumbent of the Erwin Neter Professorial Chair in Cell and Tumor Biology. We thank Dr Agnieszka Kawska (IllusScientia) for the artwork in Figure 5. Publisher Copyright: {\textcopyright} Boujemaa-Paterski et al.",
year = "2020",
month = oct,
day = "1",
doi = "10.7554/eLife.53990",
language = "English",
volume = "9",
pages = "1--26",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}