Talin-activated vinculin interacts with branched actin networks to initiate bundles

Rajaa Boujemaa-Paterski, Bruno Martins, Matthias Eibauer, Charlie T. Beales, Benjamin Geiger, Ohad Medalia

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Vinculin plays a fundamental role in integrin-mediated cell adhesion. Activated by talin, it interacts with diverse adhesome components, enabling mechanical coupling between the actin cytoskeleton and the extracellular matrix. Here we studied the interactions of activated full-length vinculin with actin and the way it regulates the organization and dynamics of the Arp2/3 complex-mediated branched actin network. Through a combination of surface patterning and light microscopy experiments we show that vinculin can bundle dendritic actin networks through rapid binding and filament crosslinking. We show that vinculin promotes stable but flexible actin bundles having a mixed-polarity organization, as confirmed by cryo-electron tomography. Adhesion-like synthetic design of vinculin activation by surface-bound talin revealed that clustered vinculin can initiate and immobilize bundles from mobile Arp2/3-branched networks. Our results provide a molecular basis for coordinate actin bundle formation at nascent adhesions.

Original languageEnglish
Article numbere53990
Pages (from-to)1-26
Number of pages26
JournaleLife
Volume9
DOIs
StatePublished - 1 Oct 2020
Externally publishedYes

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