Tandem catalysis using an enzyme and a polymeric ruthenium-based artificial metalloenzyme

Edzna S. Garcia; Thao M. Xiong; Abygail Lifschitz; Steven C. Zimmerman

doi:10.1039/d1py01255j

Tandem catalysis using an enzyme and a polymeric ruthenium-based artificial metalloenzyme

Edzna S. Garcia, Thao M. Xiong, Abygail Lifschitz, Steven C. Zimmerman

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

A Ru-containing single chain nanoparticle (SCNP) was prepared in three steps using radical polymerization of pentafluorophenylacrylate, post-polymerization functionalization with three different alkylamines, and coordination of Ru. The polymer was characterized by 1H NMR, 19F NMR, UV-vis, and DLS. The catalytic activity of the Ru-SCNP for Ru-catalyzed cleavage of allylcarbamates was evaluated by fluorescence spectroscopy and a higher percent conversion and initial rate of reaction was observed when compared to that of the free catalyst in buffer and cell media. The catalytic SCNP was also shown to perform tandem catalysis with β-galactosidase.

Original language	English
Pages (from-to)	6755-6760
Number of pages	6
Journal	Polymer Chemistry
Volume	12
Issue number	46
DOIs	https://doi.org/10.1039/d1py01255j
State	Published - 14 Dec 2021
Externally published	Yes

ASJC Scopus subject areas

Bioengineering
Biochemistry
Organic Chemistry
Polymers and Plastics

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title = "Tandem catalysis using an enzyme and a polymeric ruthenium-based artificial metalloenzyme",

abstract = "A Ru-containing single chain nanoparticle (SCNP) was prepared in three steps using radical polymerization of pentafluorophenylacrylate, post-polymerization functionalization with three different alkylamines, and coordination of Ru. The polymer was characterized by 1H NMR, 19F NMR, UV-vis, and DLS. The catalytic activity of the Ru-SCNP for Ru-catalyzed cleavage of allylcarbamates was evaluated by fluorescence spectroscopy and a higher percent conversion and initial rate of reaction was observed when compared to that of the free catalyst in buffer and cell media. The catalytic SCNP was also shown to perform tandem catalysis with β-galactosidase.",

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T1 - Tandem catalysis using an enzyme and a polymeric ruthenium-based artificial metalloenzyme

AU - Garcia, Edzna S.

AU - Xiong, Thao M.

AU - Lifschitz, Abygail

AU - Zimmerman, Steven C.

PY - 2021/12/14

Y1 - 2021/12/14

N2 - A Ru-containing single chain nanoparticle (SCNP) was prepared in three steps using radical polymerization of pentafluorophenylacrylate, post-polymerization functionalization with three different alkylamines, and coordination of Ru. The polymer was characterized by 1H NMR, 19F NMR, UV-vis, and DLS. The catalytic activity of the Ru-SCNP for Ru-catalyzed cleavage of allylcarbamates was evaluated by fluorescence spectroscopy and a higher percent conversion and initial rate of reaction was observed when compared to that of the free catalyst in buffer and cell media. The catalytic SCNP was also shown to perform tandem catalysis with β-galactosidase.

AB - A Ru-containing single chain nanoparticle (SCNP) was prepared in three steps using radical polymerization of pentafluorophenylacrylate, post-polymerization functionalization with three different alkylamines, and coordination of Ru. The polymer was characterized by 1H NMR, 19F NMR, UV-vis, and DLS. The catalytic activity of the Ru-SCNP for Ru-catalyzed cleavage of allylcarbamates was evaluated by fluorescence spectroscopy and a higher percent conversion and initial rate of reaction was observed when compared to that of the free catalyst in buffer and cell media. The catalytic SCNP was also shown to perform tandem catalysis with β-galactosidase.

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U2 - 10.1039/d1py01255j

DO - 10.1039/d1py01255j

M3 - Article

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SN - 1759-9954

VL - 12

SP - 6755

EP - 6760

JO - Polymer Chemistry

JF - Polymer Chemistry

IS - 46

ER -

Tandem catalysis using an enzyme and a polymeric ruthenium-based artificial metalloenzyme

Abstract

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