Tandem repeats in a new toxin gene from Bacillus thuringiensis and in other cry11-like genes

Olga Melnikov, Nadine Baranes, Monica Einav, Eitan Ben-Dov, Robert Manasherob, Mark Itsko, Arieh Zaritsky

Research output: Contribution to journalArticlepeer-review

Abstract

A new gene, cry11Bb2 from a field isolate of Bacillus thuringiensis, was cloned for expression in Escherichia coli. The encoded protein, with a deduced molecular mass of 89.5 kDa, exhibits 97 and 79% identities with the overlap regions of Cry11Bb1 from B. thuringiensis ssp. medellin and Cry11Ba1 from ssp. jegathesan, respectively. It is however longer than Cry11Bb1 by 42 amino acids in its carboxy-terminus, of which 32 comprise 2 tandem repeats additional to the 5 existing in the latter polypeptide. Possible roles for this recurrent motif among Cry toxins and their accessory proteins, and for their encoding genes are proposed.

Original languageEnglish
Pages (from-to)204-210
Number of pages7
JournalJournal of Molecular Microbiology and Biotechnology
Volume20
Issue number4
DOIs
StatePublished - 1 Oct 2011

Keywords

  • Bacillus thuringiensis, a field isolate
  • Mosquito larvae
  • Tandem repeats
  • cry11Bb2

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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