Taurine Stabilizing Effect on Lysozyme

Leonardo Mastrella, Paolo Moretti, Silvia Pieraccini, Simona Magi, Silvia Piccirillo, Maria Grazia Ortore

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Taurine is an important organic osmolyte in mammalian cells, and it weakens inflammation and oxidative stress mediated injuries in some diseases. Recently, taurine has been demonstrated to play a therapeutic role against neurodegenerative disorders, although its parallel involvement in several biochemical mechanisms makes not clear taurine specific role in these diseases. Furthermore, the stabilizing effect of this molecule in terms of protein stability is known, but not deeply investigated. In this work we explore by Circular Dichroism the stabilizing impact of taurine in lysozyme thermal denaturation and its influence in lysozyme aggregation into amyloid fibrils. Taurine even at low concentration modifies protein-protein interactions in lysozyme native state, as revealed by Small Angle X-ray Scattering experiments, and alters the amyloid aggregation pattern without completely inhibiting it, as confirmed by UV/Vis spectroscopy with Congo Red and by Atomic Force Microscopy. Evaluation of the cytotoxicities of the amyloid fibrils grown in presence or in absence of taurine is investigated on SH-SY5Y neuroblastoma cells.

Original languageEnglish
Article number133
Issue number1
StatePublished - 1 Jan 2022
Externally publishedYes


  • Amyloid
  • Circular Dichroism
  • Lysozyme
  • Protein solvation
  • Small Angle X-ray Scattering
  • Spectroscopy
  • Taurine

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry, Genetics and Molecular Biology (all)
  • Paleontology
  • Space and Planetary Science


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