TY - JOUR
T1 - Temperature dependence of agonist binding to muscarinic receptors in rat hypothalamic regions
AU - Galron, Ronit
AU - Avissar, Sofia
AU - Sokolovsky, Mordechai
N1 - Funding Information:
Data Analysis. Theoretical binding curves were fitted to the experimental data points using the nonlinear least square regression computer program BMDPAR, revision date November 1978, as described in detail previously (1)(4)(5). (The program was developed at the Health Science Computing Facility of the University of California, Los Angeles. The facility is sponsored by NIH Special Research Resources Grant R-R-3). Statistical evaluations of agonist binding parameters (affinity constants and population of high affinity sites) were made using Student's t test.
Funding Information:
Enlightening discussions with Dr. Yoav Henis are gratefully acknowledged. Ms. Talia Ben-Meir provided excellent technical assisstance. This work was supported in part by the Recanati Foundation for Medical Research (Israel).
PY - 1981/9/30
Y1 - 1981/9/30
N2 - The temperature dependence of the muscarinic binding characteristics in various brain regions was investigated at 25° and 37°. An unusual degree of temperature dependence was observed for agonist binding parameters in the hypothalamus, especially in the preoptic area. We therefore chose this region for a more detailed study over a temperature range of 25°-42°. Changes due to temperature alterations in the proportion of high-affinity sites, as well as in the high-affinity state constant, were found in male rats, while in females at the proestrous stage only the high-affinity constant was affected. The possible involvement of the muscarinic system is discussed in light of the well known role of the hypothalamus in body thermoregulation.
AB - The temperature dependence of the muscarinic binding characteristics in various brain regions was investigated at 25° and 37°. An unusual degree of temperature dependence was observed for agonist binding parameters in the hypothalamus, especially in the preoptic area. We therefore chose this region for a more detailed study over a temperature range of 25°-42°. Changes due to temperature alterations in the proportion of high-affinity sites, as well as in the high-affinity state constant, were found in male rats, while in females at the proestrous stage only the high-affinity constant was affected. The possible involvement of the muscarinic system is discussed in light of the well known role of the hypothalamus in body thermoregulation.
UR - http://www.scopus.com/inward/record.url?scp=0019767122&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(81)80201-X
DO - 10.1016/S0006-291X(81)80201-X
M3 - Article
C2 - 7306184
AN - SCOPUS:0019767122
SN - 0006-291X
VL - 102
SP - 791
EP - 798
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -