Temperature dependent lipase production from cold and pH tolerant species of Penicillium

N. Pandey, K. Dhakar, R. Jain, Anita Pandey

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

The psychrotolerant microorganisms are receiving attention of the scientific community due to their ability to produce biotechnological products. The present study is focused on the diversity of cold and pH tolerant isolates of Penicillium spp with respect to their potential to produce cold active lipases. The characterization of the fungal isolates was done using polyphasic approach (morphological and molecular methods). The isolates were found to have tolerance for temperature from 4-35 °C (opt.21-25 °C) and pH 2-14 (opt. 5-7). Lipase production was investigated under the influence of temperature between 5-35 °C. The fungal isolates were found to produce lipase, optimally at different temperatures, up to 25 days of incubation. Maximum lipase production was recorded at 15 and 25 °C temperatures, whereas it was minimum at 5 and 35 °C. Three fungal isolates, designated as GBPI_P98, GBPI_P150 and GBPI_P228, were found to produce optimal lipase at 25 °C whereas seven isolates, GBPI_P8, GBPI_P36, GBPI_P72, GBPI_P101, GBPI_P141, GBPI_P188 and GBPI_P222, showed maximum lipase prodution at 15 °C. In general, production of biomass showed no relation with the lipase activity. The study will have inference in production of cold active lipase for their versatile uses in biotechnological industries.

Original languageEnglish
Pages (from-to)1533-1545
Number of pages13
JournalMycosphere
Volume7
Issue number10
DOIs
StatePublished - 1 Jan 2016
Externally publishedYes

Keywords

  • Fungi
  • Indian Himalayan region
  • Lipase
  • Penicillium
  • Psychrotolerants

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science

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