Temperature effects of excitation laser pulses during step-scan FT-IR measurements

C. Rödig; H. Georg; F. Siebert; I. Rousso; M. Sheves

doi:10.1155/1999/50762

Temperature effects of excitation laser pulses during step-scan FT-IR measurements

C. Rödig, H. Georg, F. Siebert, I. Rousso, M. Sheves

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Replacing the chromophore of bacteriorhodopsin with chemically modified retinal analogs which cannot isomerize, allows to measure directly side effects from the laser pulse used for sample excitation in step-scan FT-IR measurements. Comparison with static temperature difference spectra and temperature-jump experiments shows that the observed effects can mainly be attributed to heating of the sample by the laser pulse. The size of resulting spectral changes is compared to difference bands of the photoreaction.

Original language	English
Pages (from-to)	169-172
Number of pages	4
Journal	Laser Chemistry
Volume	19
Issue number	1-4
DOIs	https://doi.org/10.1155/1999/50762
State	Published - 1 Jan 1999
Externally published	Yes

Keywords

Bacteriorhodopsin
Locked retinal analogs
Step-scan FT-IR spectroscopy

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Biochemistry
Spectroscopy

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10.1155/1999/50762

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abstract = "Replacing the chromophore of bacteriorhodopsin with chemically modified retinal analogs which cannot isomerize, allows to measure directly side effects from the laser pulse used for sample excitation in step-scan FT-IR measurements. Comparison with static temperature difference spectra and temperature-jump experiments shows that the observed effects can mainly be attributed to heating of the sample by the laser pulse. The size of resulting spectral changes is compared to difference bands of the photoreaction.",

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AU - Rödig, C.

AU - Georg, H.

AU - Siebert, F.

AU - Rousso, I.

AU - Sheves, M.

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Replacing the chromophore of bacteriorhodopsin with chemically modified retinal analogs which cannot isomerize, allows to measure directly side effects from the laser pulse used for sample excitation in step-scan FT-IR measurements. Comparison with static temperature difference spectra and temperature-jump experiments shows that the observed effects can mainly be attributed to heating of the sample by the laser pulse. The size of resulting spectral changes is compared to difference bands of the photoreaction.

AB - Replacing the chromophore of bacteriorhodopsin with chemically modified retinal analogs which cannot isomerize, allows to measure directly side effects from the laser pulse used for sample excitation in step-scan FT-IR measurements. Comparison with static temperature difference spectra and temperature-jump experiments shows that the observed effects can mainly be attributed to heating of the sample by the laser pulse. The size of resulting spectral changes is compared to difference bands of the photoreaction.

KW - Bacteriorhodopsin

KW - Locked retinal analogs

KW - Step-scan FT-IR spectroscopy

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U2 - 10.1155/1999/50762

DO - 10.1155/1999/50762

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ER -

Temperature effects of excitation laser pulses during step-scan FT-IR measurements

Abstract

Keywords

ASJC Scopus subject areas

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