Temperature effects of excitation laser pulses during step-scan FT-IR measurements

C. Rödig, H. Georg, F. Siebert, I. Rousso, M. Sheves

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Replacing the chromophore of bacteriorhodopsin with chemically modified retinal analogs which cannot isomerize, allows to measure directly side effects from the laser pulse used for sample excitation in step-scan FT-IR measurements. Comparison with static temperature difference spectra and temperature-jump experiments shows that the observed effects can mainly be attributed to heating of the sample by the laser pulse. The size of resulting spectral changes is compared to difference bands of the photoreaction.

Original languageEnglish
Pages (from-to)169-172
Number of pages4
JournalLaser Chemistry
Volume19
Issue number1-4
DOIs
StatePublished - 1 Jan 1999
Externally publishedYes

Keywords

  • Bacteriorhodopsin
  • Locked retinal analogs
  • Step-scan FT-IR spectroscopy

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Biochemistry
  • Spectroscopy

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