Temperature‐induced expression of proteins in Leishmania mexicana amazonensis A 22‐kDa protein is possibly localized in the mitochondrion

Elena PINELLI, Michal SHAPIRA

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Temperature increase is an integral part of Leishmania life cycle, and plays a major role in stage transformation. Analysis of the temperature‐dependent pattern of protein synthesis on two‐dimensional gel electrophoresis shows that, in addition to the conserved heat‐shock type of response in which expression of the major 70‐kDa and 83‐kDa heat‐shock proteins is observed, a group of low‐molecular‐mass (17–40 kDa) proteins is induced in promastigotes of Leishmania mexicana amazonensis at elevated temperatures. Immuno‐gold labelling with antibodies raised against the heat‐induced 22‐kDa proteins was localized mainly in the mitochondrion of Leishmania parasites, though labelling was observed also in the nucleus. The correlation of this finding with various reports on induction of mitochondrial enzymes in response to temperature stress in other organisms is discussed.

Original languageEnglish
Pages (from-to)685-691
Number of pages7
JournalEuropean Journal of Biochemistry
Volume194
Issue number2
DOIs
StatePublished - 1 Jan 1990
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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