Tetrabutylammonium fluoride-mediated rapid alkylation reaction in microtiter plates for the discovery of enzyme inhibitors in situ

Chung Yi Wu, Ashraf Brik, Sheng Kai Wang, Yu Hsien Chen, Chi Huey Wong

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

(Chemical Equation Presented) No isolation, no purification. An efficient and simple method based on tetrabutylammonium fluoride-mediated alkylation without protecting groups in microtiter plates, followed by screening in situ has been developed to identify new enzyme inhibitors, as demonstrated in the discovery of the shown potent inhibitors of cathepsin B and HIV protease.

Original languageEnglish
Pages (from-to)2176-2180
Number of pages5
JournalChemBioChem
Volume6
Issue number12
DOIs
StatePublished - 1 Dec 2005
Externally publishedYes

Keywords

  • Alkylation
  • Chemistry esterification
  • Combinatorial
  • Inhibitors
  • TBAF

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