The activation of β2-adrenergic receptors in naïve rats causes a reduction of blood glutamate levels: Relevance to stress and neuroprotection

Alexander Zlotnik, Yael Klin, Benjamin F. Gruenbaum, Shaun E. Gruenbaum, Sharon Ohayon, Mathew Boyko, Eyal Sheiner, Barak Aricha-Tamir, Yoram Shapira, Vivian I. Teichberg

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

This study examines the effects of the activation of β1 and β2-adrenergic receptors on glutamate homeostasis in the blood of naïve rats. Forty five male Sprague-Dawley rats were randomly assigned into one of seven treatment groups that were treated with various β-adrenergic receptor agonist and antagonist drugs. Blood glutamate levels were determined at t = 0, 30, 60, 90, and 120 min. The activation of β1 and β2-adrenergic receptors via isoproterenol hydrochloride administration produced a marked sustained decrease in blood glutamate levels by 60 min after treatment (ANOVA, t = 60, 90 min: P < 0.05, t = 120 min: P < 0.01). Pretreatment with propranolol hydrochloride (a non-selective β-adrenergic receptor blocker) or butaxamine hydrochloride (a selective β2-adrenergic receptor blocker) occluded the isoproterenol-mediated decrease in blood glutamate levels. Propranolol alone had no effect on blood glutamate levels. Selective β1-adrenergic receptor blockade with metoprolol resulted in decreased blood glutamate levels (ANOVA, t = 90 min: P < 0.05, t = 120 min: P < 0.01). Butaxamine hydrochloride alone resulted in a delayed-onset increase in glutamate levels (ANOVA, t = 120 min: P < 0.05). The results suggest that the activation of β2 receptors plays an important role in the homeostasis of glutamate in rat blood.

Original languageEnglish
Pages (from-to)732-738
Number of pages7
JournalNeurochemical Research
Volume36
Issue number5
DOIs
StatePublished - 1 May 2011

Keywords

  • Glutamate
  • Recovery
  • Traumatic brain injury
  • β2-Adrenergic receptors

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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