The cleavage of transfer RNA by a single strand specific endonuclease from neurospora crassa

Jacov Tal

doi:10.1093/nar/2.7.1073

The cleavage of transfer RNA by a single strand specific endonuclease from neurospora crassa

Jacov Tal

Research output: Contribution to journal › Article › peer-review

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Abstract

Endonuclease from Neurospora crassa (NcNase), an enzyme with specificity for polynucleotides lacking an ordered structure, was shown to cleave sut su⁺₃ tRNA^Tyr from E. coli preferentially in the anticodon region. The enzyme cleaved unfractionated tRNA primarily to 30 - 50 nucleotide size fragments, implying that most or all tRNA species are also cleaveH in the anticodon region. The 3′ terminal sequence C-A was cleaved as well. The results are discussed with respect to the three dimensional structure of tRNA.

Original language	English
Pages (from-to)	1073-1082
Number of pages	10
Journal	Nucleic Acids Research
Volume	2
Issue number	7
DOIs	https://doi.org/10.1093/nar/2.7.1073
State	Published - 1 Jul 1975
Externally published	Yes

ASJC Scopus subject areas

Genetics

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title = "The cleavage of transfer RNA by a single strand specific endonuclease from neurospora crassa",

abstract = "Endonuclease from Neurospora crassa (NcNase), an enzyme with specificity for polynucleotides lacking an ordered structure, was shown to cleave sut su+3 tRNATyr from E. coli preferentially in the anticodon region. The enzyme cleaved unfractionated tRNA primarily to 30 - 50 nucleotide size fragments, implying that most or all tRNA species are also cleaveH in the anticodon region. The 3′ terminal sequence C-A was cleaved as well. The results are discussed with respect to the three dimensional structure of tRNA.",

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AB - Endonuclease from Neurospora crassa (NcNase), an enzyme with specificity for polynucleotides lacking an ordered structure, was shown to cleave sut su+3 tRNATyr from E. coli preferentially in the anticodon region. The enzyme cleaved unfractionated tRNA primarily to 30 - 50 nucleotide size fragments, implying that most or all tRNA species are also cleaveH in the anticodon region. The 3′ terminal sequence C-A was cleaved as well. The results are discussed with respect to the three dimensional structure of tRNA.

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The cleavage of transfer RNA by a single strand specific endonuclease from neurospora crassa

Abstract

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