The cleavage of transfer RNA by a single strand specific endonuclease from neurospora crassa

Jacov Tal

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Endonuclease from Neurospora crassa (NcNase), an enzyme with specificity for polynucleotides lacking an ordered structure, was shown to cleave sut su+3 tRNATyr from E. coli preferentially in the anticodon region. The enzyme cleaved unfractionated tRNA primarily to 30 - 50 nucleotide size fragments, implying that most or all tRNA species are also cleaveH in the anticodon region. The 3′ terminal sequence C-A was cleaved as well. The results are discussed with respect to the three dimensional structure of tRNA.

Original languageEnglish
Pages (from-to)1073-1082
Number of pages10
JournalNucleic Acids Research
Volume2
Issue number7
DOIs
StatePublished - 1 Jul 1975
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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