Abstract
Endonuclease from Neurospora crassa (NcNase), an enzyme with specificity for polynucleotides lacking an ordered structure, was shown to cleave sut su+3 tRNATyr from E. coli preferentially in the anticodon region. The enzyme cleaved unfractionated tRNA primarily to 30 - 50 nucleotide size fragments, implying that most or all tRNA species are also cleaveH in the anticodon region. The 3′ terminal sequence C-A was cleaved as well. The results are discussed with respect to the three dimensional structure of tRNA.
Original language | English |
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Pages (from-to) | 1073-1082 |
Number of pages | 10 |
Journal | Nucleic Acids Research |
Volume | 2 |
Issue number | 7 |
DOIs | |
State | Published - 1 Jul 1975 |
Externally published | Yes |
ASJC Scopus subject areas
- Genetics