The complete coding sequence of the human a-raf-1 oncogene and transforming activity of a human a-raf carrying retrovirus

T. W. Beck; M. Huleihel; M. Gunnell; T. I. Bonner; U. R. Rapp

doi:10.1093/nar/15.2.595

The complete coding sequence of the human a-raf-1 oncogene and transforming activity of a human a-raf carrying retrovirus

T. W. Beck, M. Huleihel, M. Gunnell, T. I. Bonner, U. R. Rapp

Research output: Contribution to journal › Article › peer-review

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Abstract

The complete 606 amino acid sequence of the human A-raf oncogene has been deduced from the 2453 nucleotide sequence of a human T cell cDNA. A cysteine-rich region located near the amino terminus, which is highly conserved in A-raf and c-raf. shows significant homology with protein kinase C. A 5′ deleted fragment of the cDNA has been incorporated into a murine retrovirus which endows the virus with the ability to transform cells in vivo and in vitro. Functionally, human A-raf is similar to v-raf and v-mos in that transformation is independent of ras gene function.

Original language	English
Pages (from-to)	595-609
Number of pages	15
Journal	Nucleic Acids Research
Volume	15
Issue number	2
DOIs	https://doi.org/10.1093/nar/15.2.595
State	Published - 26 Jan 1987
Externally published	Yes

ASJC Scopus subject areas

Genetics

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title = "The complete coding sequence of the human a-raf-1 oncogene and transforming activity of a human a-raf carrying retrovirus",

abstract = "The complete 606 amino acid sequence of the human A-raf oncogene has been deduced from the 2453 nucleotide sequence of a human T cell cDNA. A cysteine-rich region located near the amino terminus, which is highly conserved in A-raf and c-raf. shows significant homology with protein kinase C. A 5′ deleted fragment of the cDNA has been incorporated into a murine retrovirus which endows the virus with the ability to transform cells in vivo and in vitro. Functionally, human A-raf is similar to v-raf and v-mos in that transformation is independent of ras gene function.",

author = "Beck, {T. W.} and M. Huleihel and M. Gunnell and Bonner, {T. I.} and Rapp, {U. R.}",

note = "Funding Information: ACKNOWLEDGEMENTS We thank Patricia Borchert, Stephen Kerby and Robert Nalewaik for excellent technical assistance, M.R. Smith and D.W. Stacey for the microinjection work, and Walter Kolch for helpful discussions. This project was funded in part by the Department of Health and Human Services under contract no. NOl-CO-23910 with Program Resources, Inc.",

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AU - Bonner, T. I.

AU - Rapp, U. R.

N1 - Funding Information: ACKNOWLEDGEMENTS We thank Patricia Borchert, Stephen Kerby and Robert Nalewaik for excellent technical assistance, M.R. Smith and D.W. Stacey for the microinjection work, and Walter Kolch for helpful discussions. This project was funded in part by the Department of Health and Human Services under contract no. NOl-CO-23910 with Program Resources, Inc.

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AB - The complete 606 amino acid sequence of the human A-raf oncogene has been deduced from the 2453 nucleotide sequence of a human T cell cDNA. A cysteine-rich region located near the amino terminus, which is highly conserved in A-raf and c-raf. shows significant homology with protein kinase C. A 5′ deleted fragment of the cDNA has been incorporated into a murine retrovirus which endows the virus with the ability to transform cells in vivo and in vitro. Functionally, human A-raf is similar to v-raf and v-mos in that transformation is independent of ras gene function.

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The complete coding sequence of the human a-raf-1 oncogene and transforming activity of a human a-raf carrying retrovirus

Abstract

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