TY - JOUR
T1 - The complete sequence of Drosophila alpha-spectrin
T2 - Conservation of structural domains between alpha-spectrins and alpha-actinin
AU - Dubreuil, R. R.
AU - Byers, T. J.
AU - Sillman, A. L.
AU - Bar-Zvi, D.
AU - Goldstein, L. S.B.
AU - Branton, D.
PY - 1989/1/1
Y1 - 1989/1/1
N2 - We report the complete sequence of Drosophila alpha-spectrin and show that it is similar to vertebrate nonerythroid spectrins. As in vertebrates, the alpha subunit consists of two large domains of repetitive sequence (segments 109 and 11-19) separated by a short nonrepetitive sequence (segment 10). The 106-residue repetitive segments are defined by a consensus sequence of 54 residues. Chicken alpha-spectrin (Wasenius, V.-M., M. Saraste, P. Salven, M. Eramaa, L. Holm, V.-P. Lehto. 1989. J. Cell Biol. 108:79-93) shares 50 of these consensus positions. Through comparison of spectrin and alpha-actinin sequences, we describe a second lineage of spectrin segments (20 and 21) that differs from the 106-residue segments by an 8-residue insertion and by lack of many of the consensus residues. We present a model of spectrin evolution in which the repetitive lineage of segments found in spectrin and alpha-actinin arose by separate multiplication events.
AB - We report the complete sequence of Drosophila alpha-spectrin and show that it is similar to vertebrate nonerythroid spectrins. As in vertebrates, the alpha subunit consists of two large domains of repetitive sequence (segments 109 and 11-19) separated by a short nonrepetitive sequence (segment 10). The 106-residue repetitive segments are defined by a consensus sequence of 54 residues. Chicken alpha-spectrin (Wasenius, V.-M., M. Saraste, P. Salven, M. Eramaa, L. Holm, V.-P. Lehto. 1989. J. Cell Biol. 108:79-93) shares 50 of these consensus positions. Through comparison of spectrin and alpha-actinin sequences, we describe a second lineage of spectrin segments (20 and 21) that differs from the 106-residue segments by an 8-residue insertion and by lack of many of the consensus residues. We present a model of spectrin evolution in which the repetitive lineage of segments found in spectrin and alpha-actinin arose by separate multiplication events.
UR - http://www.scopus.com/inward/record.url?scp=0024449311&partnerID=8YFLogxK
U2 - 10.1083/jcb.109.5.2197
DO - 10.1083/jcb.109.5.2197
M3 - Article
C2 - 2808524
AN - SCOPUS:0024449311
SN - 0021-9525
VL - 109
SP - 2197
EP - 2205
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 5
ER -