TY - JOUR
T1 - The concentration of surfactant protein-A in amniotic fluid decreases in spontaneous human parturition at term
AU - Chaiworapongsa, Tinnakorn
AU - Hong, Joon Seok
AU - Hull, William M.
AU - Kim, Chong Jai
AU - Gomez, Ricardo
AU - Mazor, Moshe
AU - Romero, Roberto
AU - Whitsett, Jeffrey A.
N1 - Funding Information:
This research was supported in part by the Intramural Research Program of the Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, DHHS.
PY - 2008/10/8
Y1 - 2008/10/8
N2 - Objective. The fetus is thought to play a central role in the onset of labor. Pulmonary surfactant protein (SP)-A, secreted by the maturing fetal lung, has been implicated in the mechanisms initiating parturition in mice. The present study was conducted to determine whether amniotic fluid concentrations of SP-A and SP-B change during human parturition. Study design. Amniotic fluid SP-A and SP-B concentrations were measured with a sensitive and specific ELISA in the following groups of pregnant women: (1) mid-trimester of pregnancy, between 15 and 18 weeks of gestation (n = 29), (2) term pregnancy not in labor (n = 28), and (3) term pregnancy in spontaneous labor (n = 26). Non-parametric statistics were used for analysis. Results. SP-A was detected in all amniotic fluid samples. SP-B was detected in 24.1% (7/29) of mid-trimester samples and in all samples at term. The median amniotic fluid concentrations of SP-A and SP-B were significantly higher in women at term than in women in the mid-trimester (SP-A term no labor: median 5.6 μg/mL, range 2.2-15.2 μg/mL vs. mid-trimester: median 1.64 μg/mL, range 0.1-4.7 μg/mL, and SP-B term no labor: median 0.54 μg/mL, range 0.17-1.99 μg/mL vs. mid-trimester: median 0 μg/mL, range 0-0.35 μg/mL; both p < 0.001). The median amniotic fluid SP-A concentration in women at term in labor was significantly lower than that in women at term not in labor (term in labor: median 2.7 μg/mL, range 1.2-10.1 μg/mL vs. term no labor: median 5.6 μg/mL, range 2.2-15.2 μg/mL; p < 0.001). There was no significant difference in the median amniotic fluid SP-B concentrations between women in labor and those not in labor (term in labor: median 0.47 μg/mL, range 0.04-1.32 μg/mL vs. term no labor: median 0.54 μg/mL, range 0.17-1.99 μg/mL; p = 0.2). Conclusion. The amniotic fluid concentration of SP-A decreases in spontaneous human parturition at term.
AB - Objective. The fetus is thought to play a central role in the onset of labor. Pulmonary surfactant protein (SP)-A, secreted by the maturing fetal lung, has been implicated in the mechanisms initiating parturition in mice. The present study was conducted to determine whether amniotic fluid concentrations of SP-A and SP-B change during human parturition. Study design. Amniotic fluid SP-A and SP-B concentrations were measured with a sensitive and specific ELISA in the following groups of pregnant women: (1) mid-trimester of pregnancy, between 15 and 18 weeks of gestation (n = 29), (2) term pregnancy not in labor (n = 28), and (3) term pregnancy in spontaneous labor (n = 26). Non-parametric statistics were used for analysis. Results. SP-A was detected in all amniotic fluid samples. SP-B was detected in 24.1% (7/29) of mid-trimester samples and in all samples at term. The median amniotic fluid concentrations of SP-A and SP-B were significantly higher in women at term than in women in the mid-trimester (SP-A term no labor: median 5.6 μg/mL, range 2.2-15.2 μg/mL vs. mid-trimester: median 1.64 μg/mL, range 0.1-4.7 μg/mL, and SP-B term no labor: median 0.54 μg/mL, range 0.17-1.99 μg/mL vs. mid-trimester: median 0 μg/mL, range 0-0.35 μg/mL; both p < 0.001). The median amniotic fluid SP-A concentration in women at term in labor was significantly lower than that in women at term not in labor (term in labor: median 2.7 μg/mL, range 1.2-10.1 μg/mL vs. term no labor: median 5.6 μg/mL, range 2.2-15.2 μg/mL; p < 0.001). There was no significant difference in the median amniotic fluid SP-B concentrations between women in labor and those not in labor (term in labor: median 0.47 μg/mL, range 0.04-1.32 μg/mL vs. term no labor: median 0.54 μg/mL, range 0.17-1.99 μg/mL; p = 0.2). Conclusion. The amniotic fluid concentration of SP-A decreases in spontaneous human parturition at term.
KW - Amniotic fluid
KW - Labor
KW - Parturition
KW - SP-A
KW - SP-B
KW - Surfactant protein
KW - Term
UR - http://www.scopus.com/inward/record.url?scp=53149133130&partnerID=8YFLogxK
U2 - 10.1080/14767050802215193
DO - 10.1080/14767050802215193
M3 - Article
C2 - 18828058
AN - SCOPUS:53149133130
SN - 1476-7058
VL - 21
SP - 652
EP - 659
JO - Journal of Maternal-Fetal and Neonatal Medicine
JF - Journal of Maternal-Fetal and Neonatal Medicine
IS - 9
ER -