TY - JOUR
T1 - The COP9 complex, a novel multisubunit nuclear regulator involved in light control of a plant developmental switch
AU - Chamovitz, Daniel A.
AU - Wei, Ning
AU - Osterlund, Mark T.
AU - Von Arnim, Albrecht G.
AU - Staub, Jeffrey M.
AU - Matsui, Minami
AU - Deng, Xing Wang
N1 - Funding Information:
Correspondence should be addressed to X.-W. D. We thank Drs. Naoshi Dohmae and Koji Takio of the Division of Biomolecular Characterization (RIKEN, Japan) for assistance in protein digestion and peptide sequencing; the W. M. Keck Foundation Biotechnology Resource Laboratory at Yale University for amino acid analysis; Prof. Ethan Signer for advising in large-scale tissue homogenization; Barry Piekos for assistance with electron microscopy; and Prof. Arthur Galston for critical reading of the manuscript. This research was supported by a National Science Foundation (NSF) grant (MCB-9513366) to X.-W. D. X.-W. D. is an NSF Presidential Faculty Fellow, D. A. C. is a postdoctoral fellow of the International Human Frontier Science Program, M. T. O. is a National Institutes of Health (NIH) predoctoral trainee, and J. M. S. is a NIH postdoctoral fellow.
PY - 1996/7/12
Y1 - 1996/7/12
N2 - Arabidopsis COP9 is a component of a large protein complex that is essential for the light control of a developmental switch and whose conformation or size is modulated by light. The complex is acidic, binds heparin, and is localized within the nucleus. Biochemical purification of the complex to near homogeneity revealed that it contains 12 distinct subunits. One of the other subunits is COP11, mutations in which result in a phenotype identical to cop9 mutants. The COP9 complex may act to regulate the nuclear abundance of COP1, an established repressor of photomorphogenic development. During the biogenesis of the COP9 complex, a certain degree of prior subunit association is a prerequisite for proper nuclear translocation. Since both COP9 and COP11 have closely related human counterparts, the COP9 complex probably represents a conserved developmental regulator in higher eukaryotes.
AB - Arabidopsis COP9 is a component of a large protein complex that is essential for the light control of a developmental switch and whose conformation or size is modulated by light. The complex is acidic, binds heparin, and is localized within the nucleus. Biochemical purification of the complex to near homogeneity revealed that it contains 12 distinct subunits. One of the other subunits is COP11, mutations in which result in a phenotype identical to cop9 mutants. The COP9 complex may act to regulate the nuclear abundance of COP1, an established repressor of photomorphogenic development. During the biogenesis of the COP9 complex, a certain degree of prior subunit association is a prerequisite for proper nuclear translocation. Since both COP9 and COP11 have closely related human counterparts, the COP9 complex probably represents a conserved developmental regulator in higher eukaryotes.
UR - http://www.scopus.com/inward/record.url?scp=0030581153&partnerID=8YFLogxK
U2 - 10.1016/S0092-8674(00)80082-3
DO - 10.1016/S0092-8674(00)80082-3
M3 - Article
AN - SCOPUS:0030581153
SN - 0092-8674
VL - 86
SP - 115
EP - 121
JO - Cell
JF - Cell
IS - 1
ER -