The development and validation of a novel Nanobody-Based competitive ELISA for the detection of foot and mouth disease 3ABC antibodies in cattle

Sigal Gelkop, Ariel Sobarzo, Polina Brangel, Cécile Vincke, Ema Romão, Shlomit Fedida-Metula, Nick Strom, Irene Ataliba, Frank Norbet Mwiine, Sylvester Ochwo, Lauro Velazquez-Salinas, Rachel A. McKendry, Serge Muyldermans, Julius Julian Lutwama, Elizabeth Rieder, Victoria Yavelsky, Leslie Lobel

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Effective management of foot and mouth disease (FMD) requires diagnostic tests to distinguish between infected and vaccinated animals (DIVA). To address this need, several enzyme-linked immunosorbent assay (ELISA) platforms have been developed, however, these tests vary in their sensitivity and specificity and are very expensive for developing countries. Camelid-derived single-domain antibodies fragments so-called Nanobodies, have demonstrated great efficacy for the development of serological diagnostics. This study describes the development of a novel Nanobody-based FMD 3ABC competitive ELISA, for the serological detection of antibodies against FMD Non-Structural Proteins (NSP) in Uganda cattle herds. This in-house ELISA was validated using more than 600 sera from different Uganda districts, and virus serotype specificities. The evaluation of the performance of the assay demonstrated high diagnostic sensitivity and specificity of 94 % (95 % CI: 88.9-97.2), and 97.67 % (95 % CI: 94.15-99.36) respectively, as well as the capability to detect NSP-specific antibodies against multiple FMD serotype infections. In comparison with the commercial PrioCHECK FMDV NSP-FMD test, there was a strong concordance and high correlation and agreement in the performance of the two tests. This new developed Nanobody based FMD 3ABC competitive ELISA could clearly benefit routine disease diagnosis, the establishment of disease-free zones, and the improvement of FMD management and control in endemically complex environments, such as those found in Africa.

Original languageEnglish
Article number250
JournalFrontiers in Veterinary Science
Volume5
Issue numberOCT
DOIs
StatePublished - 12 Oct 2018

Keywords

  • Antibodies
  • ELISA
  • Foot and mouth disease
  • Nanobodies
  • Non-structural proteins

ASJC Scopus subject areas

  • Veterinary (all)

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