The dynamics of actin-based motility depend on surface parameters

Anne Bernheim-Groswasser, Sebastian Wiesner, Roy M. Golsteyn, Marie France Carlier, Cecile Sykes

Research output: Contribution to journalArticlepeer-review

194 Scopus citations

Abstract

In cells, actin polymerization at the plasma membrane is induced by the recruitment of proteins such as the Arp2/3 complex, and the zyxin/VASP complex. The physical mechanism of force generation by actin polymerization has been described theoretically using various approaches, but lacks support from experimental data. By the use of reconstituted motility medium, we find that the Wiskott-Aldrich syndrome protein (WASP) subdomain, known as VCA, is sufficient to induce actin polymerization and movement when grafted on microspheres. Changes in the surface density of VCA protein or in the microsphere diameter markedly affect the velocity regime, shifting from a continuous to a jerky movement resembling that of the mutated 'hopping' Listeria. These results highlight how simple physical parameters such as surface geometry and protein density directly affect spatially controlled actin polymerization, and play a fundamental role in actin-dependent movement.

Original languageEnglish
Pages (from-to)308-311
Number of pages4
JournalNature
Volume417
Issue number6886
DOIs
StatePublished - 1 Jan 2002
Externally publishedYes

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