The effect of acridine orange on the structure of ribosomes

Moshe Tal; Haim Rotem; Matya Alfasi; Robert A. Berg

doi:10.1002/bip.1973.360120116

The effect of acridine orange on the structure of ribosomes

Moshe Tal, Haim Rotem, Matya Alfasi, Robert A. Berg

Research output: Contribution to journal › Article › peer-review

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Abstract

Exposure of Escherichia coli MRE‐600 ribosomes to acridine orange (AO) at low ionic strength (1mM Tris‐acetate pH 7.4) results in quantitative binding of the dye. Under our experimental conditions about a few hundred dye molecules can be bound to any one of the 30, 50, or 70‐S particles. AO causes the 30 and the 50‐S subunits to form ribosomal aggregates of approximate sedimentation constants of 70 and 100‐S.

Original language	English
Pages (from-to)	173-179
Number of pages	7
Journal	Biopolymers
Volume	12
Issue number	1
DOIs	https://doi.org/10.1002/bip.1973.360120116
State	Published - 1 Jan 1973
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Biomaterials
Organic Chemistry

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The effect of acridine orange on the structure of ribosomes

Abstract

ASJC Scopus subject areas

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