A technique has been worked out for investigating rapid reactions in competitive conditions under pressure. We have studied the effect of pressure up to 1000 atm on the rate of decomposition of H2O2 by catalase. It has been found that with increase in pressure the reaction is slowed down, the effective volumetric effect of activation at atmospheric pressure of ΔV ̄≠1 is equal to +30 cm3/mole. It is concluded that the value for ΔV ̄≠1 found is due to the difference in the structure of the apoenzyme in the transitional and initial state. In the transitional state the protein has a more openwork and at the same time more rigid structure.
|Number of pages||9|
|Journal||Biophysics (Russian Federation)|
|State||Published - 1 Dec 1968|
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