The effect of ions and ionophores on cystine egress from human leucocyte lysosome-rich granular fraction

Nava Bashan, William A. Gahl, Frank Tietze, Isa Bernardini, Joseph D. Schulman

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

This paper describes the stimulation of exodus of cystine from lysosome-rich granular fractions by potassium. Potassium permeability into lysosomes is low, but in the presence of an ionophore or permeable anion, the movement of K+ into lysosomes caused a large stimulation of cystine exodus. Lysosomal preparations from leucocytes of cystinotic patients, which lack carrier-mediated cystine transport, also manifested stimulation of cystine egress by valinomycin and K+. This suggests that potassium-dependent cystine egress involves a carrier different from that defective in cystinosis, or occurs through a non-carrier-mediated mechanism.

Original languageEnglish
Pages (from-to)267-273
Number of pages7
JournalBiochimica et Biophysica Acta - Biomembranes
Volume777
Issue number2
DOIs
StatePublished - 7 Nov 1984
Externally publishedYes

Keywords

  • (Leukocyte lysosome)
  • Amino acid transport
  • Cystine efflux
  • Cystinosis
  • Ionophore

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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