The expression levels and cellular localization of pigment epithelium derived factor (PEDF) in mouse testis: Its possible involvement in the differentiation of spermatogonial cells

Noy Bagdadi, Alaa Sawaied, Ali Abumadighem, Eitan Lunenfeld, Mahmoud Huleihel

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Pigment epithelium derived factor (PEDF) is a multifunctional secretory soluble glycoprotein that belongs to the serine protease inhibitor (serpin) family. It was reported to have neurotrophic, anti-angiogenic and anti-tumorigenic activity. Recently, PEDF was found in testicular peritubular cells and it was assumed to be involved in the avascular nature of seminiferous tubules. The aim of this study was to determine the cellular origin, expression levels and target cells of PEDF in testicular tissue of immature and adult mice under physiological conditions, and to explore its possible role in the process of spermatogenesis in vitro. Using immunofluorescence staining, we showed that PEDF was localized in spermatogenic cells at different stages of development as well as in the somatic cells of the testis. Its protein levels in testicular homogenates and Sertoli cells supernatant showed a significant decrease with age. PEDF receptor (PEDF-R) was localized within the seminiferous tubule cells and in the interstitial cells compartment. Its RNA expression levels showed an increase with age until 8 weeks followed by a decrease. RNA levels of PEDF-R showed the opposite trend of the protein. Addition of PEDF to cultures of isolated cells from the seminiferous tubules did not changed their proliferation rate, however, a significant increase was observed in number of meiotic/post meiotic cells at 1000 ng/mL of PEDF; indicating an in vitro differentiation effect. This study may suggest a role for PEDF in the process of spermatogenesis.

Original languageEnglish
Article number1147
Pages (from-to)1-13
Number of pages13
JournalInternational Journal of Molecular Sciences
Volume22
Issue number3
DOIs
StatePublished - 1 Feb 2021

Keywords

  • 3D in vitro culture system
  • In vitro differentiation of spermatogonial cells
  • Male fertility
  • Pigment epithelium derived factor (PEDF)
  • Somatic cells
  • Spermatogenesis
  • Testis

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Fingerprint

Dive into the research topics of 'The expression levels and cellular localization of pigment epithelium derived factor (PEDF) in mouse testis: Its possible involvement in the differentiation of spermatogonial cells'. Together they form a unique fingerprint.

Cite this