The functional organization of layer 2-3 in the rat barrel cortex: A 2-photon imaging study

L Garion, U Dubin, Y Rubin, Y Schiller, R Azouz, J Schiller

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The barrel cortex is the primary target receiving information
from the whisker somato-sensory system. In general aliquant
cortexes are organized such that various functional feature are
spatially mapped onto the cortex. The barrel cortex has a well
organized somatotopic whisker mapp. However, despite the
fundamental importance of the whisker system in rats the
internal spatial organization of the individual barrels in general
and that of the supra-granular layers in particular are mostly
unknown. Two-photon imaging of in-vivo has a high spatial
resolution and a relatively high temporal resolution that allows
to simultaneously monitoring the activity of multiple neurons.
We used two-photon imaging of barrel cortex layer 2–3
neurons loaded with the AM form of Fluo-4 to map the spatiofunctional organization of the barrel cortex. In our experiments
we recorded the responses evoked by different sand papers
with different degrees of coarsmness (P320, P600, P1000). All
recordings were performed in the barrel of the principle
whisker, as identified by intrinsic imaging. Two-photon
imaging was performed using the free-hand line scan mode
(developed in our lab). This mode allowed to record
from an average of 54.3±3.7 neurons with temporal
resolution of 60–100 Hz. These experiments show that
both the temporal dynamics of the response and texture
coding are spatially mapped onto individual barrels. These
findings have important implications for texture coding in the
whisker barrel system
Original languageEnglish
Title of host publicationJOURNAL OF MOLECULAR NEUROSCIENCE
PagesS39-S39
Volume48
DOIs
StatePublished - 2012

Fingerprint

Dive into the research topics of 'The functional organization of layer 2-3 in the rat barrel cortex: A 2-photon imaging study'. Together they form a unique fingerprint.

Cite this