@article{b69826a76a7a432f95b1626a3ba58e1f,
title = "The genetics underlying metabolic signatures in a brown rice diversity panel and their vital role in human nutrition",
abstract = "Brown rice (Oryza sativa) possesses various nutritionally dense bioactive phytochemicals exhibiting a wide range of antioxidant, anti-cancer, and anti-diabetic properties known to promote various human health benefits. However, despite the wide claims made about the importance of brown rice for human nutrition the underlying metabolic diversity has not been systematically explored. Non-targeted metabolite profiling of developing and mature seeds of a diverse genetic panel of 320 rice cultivars allowed quantification of 117 metabolites. The metabolite genome-wide association study (mGWAS) detected genetic variants influencing diverse metabolic targets in developing and mature seeds. We further interlinked genetic variants on chromosome 7 (6.06–6.43 Mb region) with complex epistatic genetic interactions impacting multi-dimensional nutritional targets, including complex carbohydrate starch quality, the glycemic index, antioxidant catechin, and rice grain color. Through this nutrigenomics approach rare gene bank accessions possessing genetic variants in bHLH and IPT5 genes were identified through haplotype enrichment. These variants were associated with a low glycemic index, higher catechin levels, elevated total flavonoid contents, and heightened antioxidant activity in the whole grain with elevated anti-cancer properties being confirmed in cancer cell lines. This multi-disciplinary nutrigenomics approach thus allowed us to discover the genetic basis of human health-conferring diversity in the metabolome of brown rice.",
keywords = "brown rice, catechins, cytotoxicity assay, glycemic index, human nutrition, metabolite genome-wide association study (mGWAS)",
author = "Yariv Brotman and Cindy Llorente-Wiegand and Glenn Oyong and Saurabh Badoni and Gopal Misra and Roslen Anacleto and Sabiha Parween and Erstelle Pasion and Tiozon, {Rhowell N.} and Anonuevo, {Joanne J.} and deGuzman, {Maria K.} and Saleh Alseekh and Mbanjo, {Edwige G.N.} and Boyd, {Lesley A.} and Fernie, {Alisdair R.} and Nese Sreenivasulu",
note = "Funding Information: NS acknowledges funding from the RICE CGIAR Research Program, Taiwan Council of Agriculture (COA) from the Ministry of Foreign Affairs (MOFA) and the UK Biotechnology and Biological Sciences Research Council UK Research & Innovation program (Project BB/T008873/1) for their financial support. ARF and SA acknowledge funding from the PlantaSYST project by the European Union{\textquoteright}s Horizon 2020 Research and Innovation Programme (SGA‐CSA no. 664621 and no. 739582 under FPA no. 664620). We thank Anthony R. Bird for the GI service of CSIRO, Australia. We acknowledge the support of the Grain Quality and Nutrition Service Laboratory technical team, International Rice Research Institute (IRRI) for conducting the grain quality analysis. We duly acknowledge our research technicians Roldan Ilagan and Fernando Salisi for growing the diversity population in the field and greenhouse; and we thank Vito Butardo, Lenie Quiatchon‐Baeza, and Artemio Madrid Jr. for their assistance in collecting the tissue samples. We greatly appreciate the support in providing access to biocomputational facilities at the Computing and Archiving Research Environment (CoARE), Advanced Science and Technology Institute, Department of Science and Technology, Republic of the Philippines. in vitro Funding Information: NS acknowledges funding from the RICE CGIAR Research Program, Taiwan Council of Agriculture (COA) from the Ministry of Foreign Affairs (MOFA) and the UK Biotechnology and Biological Sciences Research Council UK Research & Innovation program (Project BB/T008873/1) for their financial support. ARF and SA acknowledge funding from the PlantaSYST project by the European Union{\textquoteright}s Horizon 2020 Research and Innovation Programme (SGA-CSA no. 664621 and no. 739582 under FPA no. 664620). We thank Anthony R. Bird for the in vitro GI service of CSIRO, Australia. We acknowledge the support of the Grain Quality and Nutrition Service Laboratory technical team, International Rice Research Institute (IRRI) for conducting the grain quality analysis. We duly acknowledge our research technicians Roldan Ilagan and Fernando Salisi for growing the diversity population in the field and greenhouse; and we thank Vito Butardo, Lenie Quiatchon-Baeza, and Artemio Madrid Jr. for their assistance in collecting the tissue samples. We greatly appreciate the support in providing access to biocomputational facilities at the Computing and Archiving Research Environment (CoARE), Advanced Science and Technology Institute, Department of Science and Technology, Republic of the Philippines. Publisher Copyright: {\textcopyright} 2021 Society for Experimental Biology and John Wiley & Sons Ltd",
year = "2021",
month = apr,
day = "1",
doi = "10.1111/tpj.15182",
language = "English",
volume = "106",
pages = "507--525",
journal = "Plant Journal",
issn = "0960-7412",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "2",
}