The impact of skeletal muscle contraction on CD146+Lin- pericytes

Svyatoslav Dvoretskiy, Koyal Garg, Michael Munroe, Yair Pincu, Ziad S. Mahmassani, Charlotte Coombs, Brent Blackwell, Gabriela Garcia, Garret Waterstradt, Isaac Lee, Jenny Drnevich, Justin S. Rhodes, Marni D. Boppart

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Unaccustomed resistance exercise can initiate skeletal muscle remodeling and adaptive mechanisms that can confer protection from damage and enhanced strength with subsequent stimulation. The myofiber may provide the primary origin for adaptation, yet multiple mononuclear cell types within the surrounding connective tissue may also contribute. The purpose of this study was to evaluate the acute response of muscle-resident interstitial cells to contraction initiated by electrical stimulation (e-stim) and subsequently determine the contribution of pericytes to remodeling as a result of training. Mice were subjected to bilateral e-stim or sham treatment. Following a single session of e-stim, NG2+CD45-CD31- (NG2+Lin-) pericyte, CD146+Lin- pericyte, and PDGFRα+ fibroadipogenic progenitor cell quantity and function were evaluated via multiplex flow cytometry and targeted quantitative PCR. Relative quantity was not significantly altered 24 h postcontraction, yet unique gene signatures were observed for each cell population at 3 h postcontraction. CD146+Lin- pericytes appeared to be most responsive to contraction, and upregulation of genes related to immunomodulation and extracellular matrix remodeling was observed via RNA sequencing. Intramuscular injection of CD146+Lin- pericytes did not significantly increase myofiber size yet enhanced ECM remodeling and angiogenesis in response to repeated bouts of e-stim for 4 wk. The results from this study provide the first evidence that CD146+Lin- pericytes are responsive to skeletal muscle contraction and may contribute to the beneficial outcomes associated with exercise.

Original languageEnglish
Pages (from-to)C1011-C1024
JournalAmerican Journal of Physiology - Cell Physiology
Volume317
Issue number5
DOIs
StatePublished - 1 Jan 2019
Externally publishedYes

Keywords

  • Angiogenesis
  • CD146
  • Contraction
  • Extracellular matrix
  • Fibroadipogenic progenitor
  • Pericyte
  • Remodeling
  • Stem cells

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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