The in-vitro effect of gonadotropins’ type and combination on Granulosa cells gene expressions

Yuval Yung, Adva Aizer, Sarah Tieb, Sharon Avhar Maydan, Ettie Maman, Lilach Marom Haham, Jigal Haas, Raoul Orvieto

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Objective: Nowadays, different modes and timing of GnRH-agonist combined with hCG trigger, for final follicular maturation, have been described. While LH + FSH are the naturally occurring final follicular maturation trigger, hCG is commonly use during stimulated cycle, and recently the introduction of the Dual/Double trigger combines LH + FSH + hCG. In the present study we aim to investigate the messenger RNA (mRNA) expression of reproduction-related genes in human granulosa cells (GCs) exposed to the aforementioned different types and combinations of gonadotropins. Material and methods: Mural GCs were obtained from follicular fluid aspirated during IVF protocol. GCs were seeded in culture for 4 days with daily medium exchange followed by administration of either hCG (1 U/ml); FSH (1 U/ml) and LH (8 U/ml); or hCG (1 U/ml) and FSH (1 U/ml) and LH (8 U/ml) for 16 h. mRNA was purified from harvested GCs and gene expression was quantitative by qPCR. Main outcome measures: The expression of genes related to steroidogenesis (StAR/ CYP19) and oocyte maturation (COX2/Amphiregulin) in cultured GCs. Results: The Dual/Double trigger (LH + FSH + hCG) showed higher activation of steroidogenesis (StAR/CYP19) and maturation (COX2/Amphiregulin) as compared to the naturally occurring trigger (LH + FSH) and the hCG triggers. Moreover, while the naturally occurring trigger (LH + FSH) activated maturation significantly and more intensely than the hCG trigger, no in between group differences were observed with regards to steroidogenic related genes. Conclusions: Our findings are in agreement with clinical experience, demonstrating the superiority of the double/dual (LH + FSH + hCG) trigger over the naturally occurring and the hCG triggers.

Original languageEnglish
Article number144
JournalReproductive Biology and Endocrinology
Volume20
Issue number1
DOIs
StatePublished - 1 Dec 2022
Externally publishedYes

Keywords

  • Final follicular maturation
  • Gene expression
  • Gonadotropin
  • Granulosa cells
  • IVF
  • Steroidogenesis
  • Trigger

ASJC Scopus subject areas

  • Endocrinology
  • Obstetrics and Gynecology
  • Reproductive Medicine
  • Developmental Biology

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