The involvement of ROS producing aldehyde oxidase in plant response to Tombusvirus infection

Timur M. Yergaliyev, Zhadyrassyn Nurbekova, Gulzhamal Mukiyanova, Alua Akbassova, Maxim Sutula, Sayan Zhangazin, Assyl Bari, Zhanerke Tleukulova, Malika Shamekova, Zhaksylyk K. Masalimov, Rustem T. Omarov

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

The influence of Tomato bushy stunt virus (TBSV) infection on the activity and isoformic composition of aldehyde oxidase and catalase in Nicotiana benthamiana plants was investigated. It was shown that the infection of plants with TBSV results in enhancement of leaf aldehyde oxidase (AO) isoforms AO2 and AO3. Significantly enhanced levels of superoxide radical producing activity of AO isoforms were also detected. This is the first demonstration of involvement of plant AO in defense mechanisms against viral infection. In addition, the infection caused an increased accumulation of hydrogen peroxide, compared to mock-inoculated plants. The virus infection resulted in increased activity of catalase (CAT) and superoxide dismutase (SOD) in roots and leaves of N. benthamiana. Moreover, activation of two additional CAT isoforms was observed in the leaves of plants after virus inoculation. Our findings indicate that the virus infection significantly affects enzymes responsible for the balance of ROS accumulation in plant tissue in response to pathogen attack.

Original languageEnglish
Pages (from-to)36-44
Number of pages9
JournalPlant Physiology and Biochemistry
Volume109
DOIs
StatePublished - 1 Dec 2016
Externally publishedYes

Keywords

  • Aldehyde oxidase
  • Catalase
  • Hydrogen peroxide
  • N. benthamiana
  • Reactive oxygen species
  • Superoxide dismutase
  • Tomato bushy stunt virus

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