TY - JOUR
T1 - The involvement of ROS producing aldehyde oxidase in plant response to Tombusvirus infection
AU - Yergaliyev, Timur M.
AU - Nurbekova, Zhadyrassyn
AU - Mukiyanova, Gulzhamal
AU - Akbassova, Alua
AU - Sutula, Maxim
AU - Zhangazin, Sayan
AU - Bari, Assyl
AU - Tleukulova, Zhanerke
AU - Shamekova, Malika
AU - Masalimov, Zhaksylyk K.
AU - Omarov, Rustem T.
N1 - Funding Information:
This work was supported by Kazakhstan Grant National Program 2015–2017. Foundation was provided by Ministry of Education and Science of the Republic of Kazakhstan (grant number 5344/GF4 ) within the bounds of the budgetary program 055 “Scientific and/or technical activity” and subprogram 101 “Grant financing of scientific researches”, agreement number 316 of May 13, 2016.
Publisher Copyright:
© 2016 Elsevier Masson SAS
PY - 2016/12/1
Y1 - 2016/12/1
N2 - The influence of Tomato bushy stunt virus (TBSV) infection on the activity and isoformic composition of aldehyde oxidase and catalase in Nicotiana benthamiana plants was investigated. It was shown that the infection of plants with TBSV results in enhancement of leaf aldehyde oxidase (AO) isoforms AO2 and AO3. Significantly enhanced levels of superoxide radical producing activity of AO isoforms were also detected. This is the first demonstration of involvement of plant AO in defense mechanisms against viral infection. In addition, the infection caused an increased accumulation of hydrogen peroxide, compared to mock-inoculated plants. The virus infection resulted in increased activity of catalase (CAT) and superoxide dismutase (SOD) in roots and leaves of N. benthamiana. Moreover, activation of two additional CAT isoforms was observed in the leaves of plants after virus inoculation. Our findings indicate that the virus infection significantly affects enzymes responsible for the balance of ROS accumulation in plant tissue in response to pathogen attack.
AB - The influence of Tomato bushy stunt virus (TBSV) infection on the activity and isoformic composition of aldehyde oxidase and catalase in Nicotiana benthamiana plants was investigated. It was shown that the infection of plants with TBSV results in enhancement of leaf aldehyde oxidase (AO) isoforms AO2 and AO3. Significantly enhanced levels of superoxide radical producing activity of AO isoforms were also detected. This is the first demonstration of involvement of plant AO in defense mechanisms against viral infection. In addition, the infection caused an increased accumulation of hydrogen peroxide, compared to mock-inoculated plants. The virus infection resulted in increased activity of catalase (CAT) and superoxide dismutase (SOD) in roots and leaves of N. benthamiana. Moreover, activation of two additional CAT isoforms was observed in the leaves of plants after virus inoculation. Our findings indicate that the virus infection significantly affects enzymes responsible for the balance of ROS accumulation in plant tissue in response to pathogen attack.
KW - Aldehyde oxidase
KW - Catalase
KW - Hydrogen peroxide
KW - N. benthamiana
KW - Reactive oxygen species
KW - Superoxide dismutase
KW - Tomato bushy stunt virus
UR - http://www.scopus.com/inward/record.url?scp=84986333473&partnerID=8YFLogxK
U2 - 10.1016/j.plaphy.2016.09.001
DO - 10.1016/j.plaphy.2016.09.001
M3 - Article
C2 - 27632242
AN - SCOPUS:84986333473
VL - 109
SP - 36
EP - 44
JO - Plant Physiology and Biochemistry
JF - Plant Physiology and Biochemistry
SN - 0981-9428
ER -