TY - JOUR
T1 - The metabolomic-gut-clinical axis of mankai plant-derived dietary polyphenols
AU - Yaskolka Meir, Anat
AU - Tuohy, Kieran
AU - von Bergen, Martin
AU - Krajmalnik-Brown, Rosa
AU - Heinig, Uwe
AU - Zelicha, Hila
AU - Tsaban, Gal
AU - Rinott, Ehud
AU - Kaplan, Alon
AU - Aharoni, Asaph
AU - Zeibich, Lydia
AU - Chang, Debbie
AU - Dirks, Blake
AU - Diotallevi, Camilla
AU - Arapitsas, Panagiotis
AU - Vrhovsek, Urska
AU - Ceglarek, Uta
AU - Haange, Sven Bastiaan
AU - Rolle-Kampczyk, Ulrike
AU - Engelmann, Beatrice
AU - Lapidot, Miri
AU - Colt, Monica
AU - Sun, Qi
AU - Shai, Iris
N1 - Publisher Copyright:
© 2021 by the author. Licensee MDPI, Basel, Switzerland.
PY - 2021/6/1
Y1 - 2021/6/1
N2 - Background: Polyphenols are secondary metabolites produced by plants to defend themselves from environmental stressors. We explored the effect of Wolffia globosa ‘Mankai’, a novel cultivated strain of a polyphenol-rich aquatic plant, on the metabolomic-gut clinical axis in vitro, in-vivo and in a clinical trial. Methods: We used mass-spectrometry-based metabolomics methods from three laboratories to detect Mankai phenolic metabolites and examined predicted functional pathways in a Mankai artificial-gut bioreactor. Plasma and urine polyphenols were assessed among the 294 DIRECT-PLUS 18-month trial participants, comparing the effect of a polyphenol-rich green-Mediterranean diet (+1240 mg/polyphenols/day, provided by Mankai, green tea and walnuts) to a walnuts-enriched (+440 mg/polyphenols/day) Mediterranean diet and a healthy controlled diet. Re-sults: Approximately 200 different phenolic compounds were specifically detected in the Mankai plant. The Mankai-supplemented bioreactor artificial gut displayed a significantly higher relative-abundance of 16S-rRNA bacterial gene sequences encoding for enzymes involved in phenolic com-pound degradation. In humans, several Mankai-related plasma and urine polyphenols were differ-entially elevated in the green Mediterranean group compared with the other groups (p < 0.05) after six and 18 months of intervention (e.g., urine hydroxy-phenyl-acetic-acid and urolithin-A; plasma Naringenin and 2,5-diOH-benzoic-acid). Specific polyphenols, such as urolithin-A and 4-ethylphe-nol, were directly involved with clinical weight-related changes. Conclusions: The Mankai new plant is rich in various unique potent polyphenols, potentially affecting the metabolomic-gut-clini-cal axis.
AB - Background: Polyphenols are secondary metabolites produced by plants to defend themselves from environmental stressors. We explored the effect of Wolffia globosa ‘Mankai’, a novel cultivated strain of a polyphenol-rich aquatic plant, on the metabolomic-gut clinical axis in vitro, in-vivo and in a clinical trial. Methods: We used mass-spectrometry-based metabolomics methods from three laboratories to detect Mankai phenolic metabolites and examined predicted functional pathways in a Mankai artificial-gut bioreactor. Plasma and urine polyphenols were assessed among the 294 DIRECT-PLUS 18-month trial participants, comparing the effect of a polyphenol-rich green-Mediterranean diet (+1240 mg/polyphenols/day, provided by Mankai, green tea and walnuts) to a walnuts-enriched (+440 mg/polyphenols/day) Mediterranean diet and a healthy controlled diet. Re-sults: Approximately 200 different phenolic compounds were specifically detected in the Mankai plant. The Mankai-supplemented bioreactor artificial gut displayed a significantly higher relative-abundance of 16S-rRNA bacterial gene sequences encoding for enzymes involved in phenolic com-pound degradation. In humans, several Mankai-related plasma and urine polyphenols were differ-entially elevated in the green Mediterranean group compared with the other groups (p < 0.05) after six and 18 months of intervention (e.g., urine hydroxy-phenyl-acetic-acid and urolithin-A; plasma Naringenin and 2,5-diOH-benzoic-acid). Specific polyphenols, such as urolithin-A and 4-ethylphe-nol, were directly involved with clinical weight-related changes. Conclusions: The Mankai new plant is rich in various unique potent polyphenols, potentially affecting the metabolomic-gut-clini-cal axis.
KW - Flavonoids
KW - Mediterranean diet
KW - Plant-based nutrition
KW - Polyphenols
KW - Weight loss
KW - Wolffia globosa
UR - http://www.scopus.com/inward/record.url?scp=85106758842&partnerID=8YFLogxK
U2 - 10.3390/nu13061866
DO - 10.3390/nu13061866
M3 - Article
C2 - 34070816
AN - SCOPUS:85106758842
SN - 2072-6643
VL - 13
JO - Nutrients
JF - Nutrients
IS - 6
M1 - 1866
ER -