The microRNA Transcriptome of Human Cytomegalovirus (HCMV)

Mesfin K Meshesha; Isana Veksler-Lublinsky; Ofer Isakov; Irit Reichenstein; Noam Shomron; Klara Kedem; Michal Ziv-Ukelson; Zvi Bentwich; Yonat Shemer Avni

doi:10.2174/1874357901206010038

The microRNA Transcriptome of Human Cytomegalovirus (HCMV)

Mesfin K Meshesha, Isana Veksler-Lublinsky, Ofer Isakov, Irit Reichenstein, Noam Shomron, Klara Kedem, Michal Ziv-Ukelson, Zvi Bentwich, Yonat Shemer Avni

Research output: Contribution to journal › Article › peer-review

Abstract

The purpose of the present study was to characterize the microRNA transcriptome (miRNAome) of the human cytomegalovirus (HCMV or HHV5). We used deep sequencing and real time PCR (qPCR) together with bioinformatics to analyze the pattern of small RNA expression in cells infected with low-passage isolates of HCMV as well as in plasma and amniotic fluid. We report here on the discovery of four new precursors and ten new miRNAs as well as eleven microRNA-offset-RNAs (moRs) that are all encoded by HCMV. About eighty percent of the total HCMV reads were perfectly mapped to HCMV miRNAs, strongly suggestive of their important biological role that in large remains still to be defined and characterized. Taken altogether, the results of this study demonstrate the power and usefulness of the combined bioinformatics/biological approach in discovering additional important members of HCMV- encoded small RNAs and can be applied to the study of other viruses as well.

Original language	English GB
Pages (from-to)	38-48
Journal	The open virology journal
Volume	6
DOIs	https://doi.org/10.2174/1874357901206010038
State	Published - 2012

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title = "The microRNA Transcriptome of Human Cytomegalovirus (HCMV)",

abstract = "The purpose of the present study was to characterize the microRNA transcriptome (miRNAome) of the human cytomegalovirus (HCMV or HHV5). We used deep sequencing and real time PCR (qPCR) together with bioinformatics to analyze the pattern of small RNA expression in cells infected with low-passage isolates of HCMV as well as in plasma and amniotic fluid. We report here on the discovery of four new precursors and ten new miRNAs as well as eleven microRNA-offset-RNAs (moRs) that are all encoded by HCMV. About eighty percent of the total HCMV reads were perfectly mapped to HCMV miRNAs, strongly suggestive of their important biological role that in large remains still to be defined and characterized. Taken altogether, the results of this study demonstrate the power and usefulness of the combined bioinformatics/biological approach in discovering additional important members of HCMV- encoded small RNAs and can be applied to the study of other viruses as well.",

author = "Meshesha, {Mesfin K} and Isana Veksler-Lublinsky and Ofer Isakov and Irit Reichenstein and Noam Shomron and Klara Kedem and Michal Ziv-Ukelson and Zvi Bentwich and Avni, {Yonat Shemer}",

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doi = "10.2174/1874357901206010038",

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TY - JOUR

T1 - The microRNA Transcriptome of Human Cytomegalovirus (HCMV)

AU - Meshesha, Mesfin K

AU - Veksler-Lublinsky, Isana

AU - Isakov, Ofer

AU - Reichenstein, Irit

AU - Shomron, Noam

AU - Kedem, Klara

AU - Ziv-Ukelson, Michal

AU - Bentwich, Zvi

AU - Avni, Yonat Shemer

PY - 2012

Y1 - 2012

N2 - The purpose of the present study was to characterize the microRNA transcriptome (miRNAome) of the human cytomegalovirus (HCMV or HHV5). We used deep sequencing and real time PCR (qPCR) together with bioinformatics to analyze the pattern of small RNA expression in cells infected with low-passage isolates of HCMV as well as in plasma and amniotic fluid. We report here on the discovery of four new precursors and ten new miRNAs as well as eleven microRNA-offset-RNAs (moRs) that are all encoded by HCMV. About eighty percent of the total HCMV reads were perfectly mapped to HCMV miRNAs, strongly suggestive of their important biological role that in large remains still to be defined and characterized. Taken altogether, the results of this study demonstrate the power and usefulness of the combined bioinformatics/biological approach in discovering additional important members of HCMV- encoded small RNAs and can be applied to the study of other viruses as well.

AB - The purpose of the present study was to characterize the microRNA transcriptome (miRNAome) of the human cytomegalovirus (HCMV or HHV5). We used deep sequencing and real time PCR (qPCR) together with bioinformatics to analyze the pattern of small RNA expression in cells infected with low-passage isolates of HCMV as well as in plasma and amniotic fluid. We report here on the discovery of four new precursors and ten new miRNAs as well as eleven microRNA-offset-RNAs (moRs) that are all encoded by HCMV. About eighty percent of the total HCMV reads were perfectly mapped to HCMV miRNAs, strongly suggestive of their important biological role that in large remains still to be defined and characterized. Taken altogether, the results of this study demonstrate the power and usefulness of the combined bioinformatics/biological approach in discovering additional important members of HCMV- encoded small RNAs and can be applied to the study of other viruses as well.

U2 - 10.2174/1874357901206010038

DO - 10.2174/1874357901206010038

M3 - מאמר

C2 - 22715351

SN - 1874-3579

VL - 6

SP - 38

EP - 48

JO - The open virology journal

JF - The open virology journal

ER -

The microRNA Transcriptome of Human Cytomegalovirus (HCMV)

Abstract

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