The Protein Kinase C-Related PKC-L(η) Gene Product Is Localized in the Cell Nucleus

Hagar Greif, Jacob Ben-Chaim, Tova Shimon, Edna Bechor, Hagit Eldar, Etta Livneh

Research output: Contribution to journalArticlepeer-review

73 Scopus citations


The tumor promoters phorbol esters are thought to induce changes in cell growth and gene expression by direct activation of protein kinase C (PKC). However, the molecular mechanisms by which PKC molecules transduce signals into the cell nucleus are unknown. In this study, we provide evidence for a direct target for phorbol esters in the nucleus. We demonstrate that the new PKC-related family member, PKC-L, recently isolated by us, is expressed specifically in the cell nucleus. Localization of PKC-L in the cell nucleus is shown both by immunofluorescence staining and by subcellular fractionation experiments of several human cell lines, including the human epidermoid carcinoma line A431. Treatment of these cells by phorbol esters does not induce the down-regulation of PKC-L, in contrast to their effect on classical PKC family members. This is the only PKC isoenzyme described so far that resides permanently and specifically in the cell nucleus. PKC-L may function as an important link in tumor promoting, e.g., as a nuclear regulator of gene expression that changes the phosphorylation state of transcriptional components such as the AP-1 complex.

Original languageEnglish
Pages (from-to)1304-1311
Number of pages8
JournalMolecular and Cellular Biology
Issue number3
StatePublished - 1 Jan 1992
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'The Protein Kinase C-Related PKC-L(η) Gene Product Is Localized in the Cell Nucleus'. Together they form a unique fingerprint.

Cite this