The rationale for requesting a second consecutive sperm ejaculate for assisted reproductive technology

I. Bar-Hava, T. Perri, J. Ashkenazi, M. Shelef, Z. Ben-Rafael, R. Orvieto

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


We assessed the value of requesting a second semen sample provided within 1 hour of the first in cases of unacceptable ejaculate quality (compared to previous occasions) or very poor semen characteristics. The study population consisted of 109 males with severe oligoterato-astenospermic (OTA) syndrome in an assisted reproduction program. Semen volume, sperm count and sperm motility were recorded in the first and second samples with a Makler Counting Chamber before sperm processing, and total motile sperm in the ejaculate was calculated. Differences in sperm parameters between the consecutive samples were determined by paired-sample t test. In 36 cases (33%) the second sample was found to be superior in quality to the first and was therefore used for the fertilization process. Of the 12/109 cases in which the first ejaculate demonstrated no motile sperm, five (41%) had detectable motile sperm in the second (total 0.1 × 106, 0.2 × 106, 0.3 × 106, 8.4 × 106 and 20.8 × 106). We conclude that a request for second ejaculate immediately after the first in males with poor semen quality or no detectable motile sperm can yield a better sample in a significant percentage of cases. Using this method, clinicians can avoid the utilization of elaborate sperm processing techniques and the need for unnecessary micromanipulative fertilization.

Original languageEnglish
Pages (from-to)433-436
Number of pages4
JournalGynecological Endocrinology
Issue number6
StatePublished - 1 Jan 2000
Externally publishedYes


  • Assisted Reproductive Technology
  • Ejaculate
  • Semen sample

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Obstetrics and Gynecology


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