The reactivation of DnaA(L366K) requires less acidic phospholipids supporting their role in the initiation of chromosome replication in Escherichia coli

Alexander Aranovich; Abraham H. Parola; Itzhak Fishov

doi:10.1016/j.febslet.2007.08.019

The reactivation of DnaA(L366K) requires less acidic phospholipids supporting their role in the initiation of chromosome replication in Escherichia coli

Alexander Aranovich, Abraham H. Parola, Itzhak Fishov

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

DnaA(L366K), in concert with a wild-type DnaA (wtDnaA) protein, restores the growth of Escherichia coli cells arrested in the absence of adequate levels of cellular acidic phospholipids. In vitro and in vivo studies showed that DnaA(L366K) alone does not induce the initiation of replication, and wtDnaA must also be present. Hitherto the different behavior of wt and mutant DnaA were not understood. We now demonstrate that this mutant may be activated at significantly lower concentrations of acidic phospholipids than the wild-type protein, and this may explain the observed growth restoration in vivo.

Original language	English
Pages (from-to)	4439-4442
Number of pages	4
Journal	FEBS Letters
Volume	581
Issue number	23
DOIs	https://doi.org/10.1016/j.febslet.2007.08.019
State	Published - 18 Sep 2007

Keywords

Amphitropic protein
Bacterial cell cycle
Macromolecular crowding
Membrane occupancy
Protein-membrane interaction

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/j.febslet.2007.08.019

Cite this

@article{707ceed50bd34327b5890d596314b59b,

title = "The reactivation of DnaA(L366K) requires less acidic phospholipids supporting their role in the initiation of chromosome replication in Escherichia coli",

abstract = "DnaA(L366K), in concert with a wild-type DnaA (wtDnaA) protein, restores the growth of Escherichia coli cells arrested in the absence of adequate levels of cellular acidic phospholipids. In vitro and in vivo studies showed that DnaA(L366K) alone does not induce the initiation of replication, and wtDnaA must also be present. Hitherto the different behavior of wt and mutant DnaA were not understood. We now demonstrate that this mutant may be activated at significantly lower concentrations of acidic phospholipids than the wild-type protein, and this may explain the observed growth restoration in vivo.",

keywords = "Amphitropic protein, Bacterial cell cycle, Macromolecular crowding, Membrane occupancy, Protein-membrane interaction",

author = "Alexander Aranovich and Parola, {Abraham H.} and Itzhak Fishov",

note = "Funding Information: We thank Prof. E. Crooke (Georgetown University, Washington, DC) for kindly providing us with the wild-type His-tagged DnaA construct and for helpful discussions. The management of Dr. R. Cohen-Luria is most appreciated. This work was supported by the Israel Science Foundation Grant No. 512/02 (to AHP and IF) and partially by the James Frank Foundation Grant (to A.H.P.).",

year = "2007",

month = sep,

day = "18",

doi = "10.1016/j.febslet.2007.08.019",

language = "English",

volume = "581",

pages = "4439--4442",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "23",

}

TY - JOUR

T1 - The reactivation of DnaA(L366K) requires less acidic phospholipids supporting their role in the initiation of chromosome replication in Escherichia coli

AU - Aranovich, Alexander

AU - Parola, Abraham H.

AU - Fishov, Itzhak

N1 - Funding Information: We thank Prof. E. Crooke (Georgetown University, Washington, DC) for kindly providing us with the wild-type His-tagged DnaA construct and for helpful discussions. The management of Dr. R. Cohen-Luria is most appreciated. This work was supported by the Israel Science Foundation Grant No. 512/02 (to AHP and IF) and partially by the James Frank Foundation Grant (to A.H.P.).

PY - 2007/9/18

Y1 - 2007/9/18

N2 - DnaA(L366K), in concert with a wild-type DnaA (wtDnaA) protein, restores the growth of Escherichia coli cells arrested in the absence of adequate levels of cellular acidic phospholipids. In vitro and in vivo studies showed that DnaA(L366K) alone does not induce the initiation of replication, and wtDnaA must also be present. Hitherto the different behavior of wt and mutant DnaA were not understood. We now demonstrate that this mutant may be activated at significantly lower concentrations of acidic phospholipids than the wild-type protein, and this may explain the observed growth restoration in vivo.

AB - DnaA(L366K), in concert with a wild-type DnaA (wtDnaA) protein, restores the growth of Escherichia coli cells arrested in the absence of adequate levels of cellular acidic phospholipids. In vitro and in vivo studies showed that DnaA(L366K) alone does not induce the initiation of replication, and wtDnaA must also be present. Hitherto the different behavior of wt and mutant DnaA were not understood. We now demonstrate that this mutant may be activated at significantly lower concentrations of acidic phospholipids than the wild-type protein, and this may explain the observed growth restoration in vivo.

KW - Amphitropic protein

KW - Bacterial cell cycle

KW - Macromolecular crowding

KW - Membrane occupancy

KW - Protein-membrane interaction

UR - http://www.scopus.com/inward/record.url?scp=34548444511&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2007.08.019

DO - 10.1016/j.febslet.2007.08.019

M3 - Article

AN - SCOPUS:34548444511

SN - 0014-5793

VL - 581

SP - 4439

EP - 4442

JO - FEBS Letters

JF - FEBS Letters

IS - 23

ER -

The reactivation of DnaA(L366K) requires less acidic phospholipids supporting their role in the initiation of chromosome replication in Escherichia coli

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this