TY - JOUR
T1 - The relationship between endogenous oestradiol and vitamin D3 metabolites in serum and follicular fluid during ovarian stimulation for in-vitro fertilization and embryo transfer
AU - Potashnik, Gad
AU - Lunenfeld, Eitan
AU - Levitas, Eli
AU - Itskovitz, Joseph
AU - Albutiano, Sarit
AU - Yankowitz, Naomi
AU - Sonin, Yael
AU - Levy, Joseph
AU - Glezerman, Marek
AU - Shany, Shraga
PY - 1992/1/1
Y1 - 1992/1/1
N2 - The present study was undertaken to examine the effect of circulating oestradiol on serum levels of 25-hydroxyvitamin D3 (25-OHD3), 24,25-dihydroxyvitamin D3[24, 25-(OH)2D3], and 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] during gonadotrophin-induced ovarian stimulation in 10 healthy women undergoing in-vitro fertilization and embryo transfer (IVF). The presence of these metabolites in the follicular fluid was also investigated. Plasma oestradiol increased from 25 ± 3.2 (mean ± SE) pg/ml before initiation of treatment to 2563 ±328 pg/ml on the day of injection of human chorionic gonadotrophin (HCG) and 1641 ± 299 pg/ml on the day of ovum retrieval (P < 0.01). Serum levels of 1,25-(OH)2D3 increased from 32.0 ± 1.9 (mean ± SE) pg/ml to 46.6 ± 8.1 and 48.5 ± 7.7 pg/ml (P < 0.05) on the day of HCG and ovum retrieval, respectively. No changes in blood levels of 25-OHD3 and 24,25-(OH)2D3 were found. The presence of vitamin D metabolites in follicular fluid is documented herein for the first time. All three metabolites were present in the follicular fluid but were significantly lower than in the concurrent serum (P < 0.01). A highly significant correlation was found between serum and follicular fluid levels: r = 0.787, P < 0.001 for 1,25-(OH)2D3; r = 0.738, P < 0.01 for 25-OHD3; and r = 0.751, P < 0.01 for 24, 25-(OH)2D3. Our results suggest that raised levels of circulating oestradiol during gonadotrophin-induced ovarian stimulation are associated with a significant increase of serum 1,25-(OH)2D3.
AB - The present study was undertaken to examine the effect of circulating oestradiol on serum levels of 25-hydroxyvitamin D3 (25-OHD3), 24,25-dihydroxyvitamin D3[24, 25-(OH)2D3], and 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] during gonadotrophin-induced ovarian stimulation in 10 healthy women undergoing in-vitro fertilization and embryo transfer (IVF). The presence of these metabolites in the follicular fluid was also investigated. Plasma oestradiol increased from 25 ± 3.2 (mean ± SE) pg/ml before initiation of treatment to 2563 ±328 pg/ml on the day of injection of human chorionic gonadotrophin (HCG) and 1641 ± 299 pg/ml on the day of ovum retrieval (P < 0.01). Serum levels of 1,25-(OH)2D3 increased from 32.0 ± 1.9 (mean ± SE) pg/ml to 46.6 ± 8.1 and 48.5 ± 7.7 pg/ml (P < 0.05) on the day of HCG and ovum retrieval, respectively. No changes in blood levels of 25-OHD3 and 24,25-(OH)2D3 were found. The presence of vitamin D metabolites in follicular fluid is documented herein for the first time. All three metabolites were present in the follicular fluid but were significantly lower than in the concurrent serum (P < 0.01). A highly significant correlation was found between serum and follicular fluid levels: r = 0.787, P < 0.001 for 1,25-(OH)2D3; r = 0.738, P < 0.01 for 25-OHD3; and r = 0.751, P < 0.01 for 24, 25-(OH)2D3. Our results suggest that raised levels of circulating oestradiol during gonadotrophin-induced ovarian stimulation are associated with a significant increase of serum 1,25-(OH)2D3.
KW - In-vitro fertilization
KW - Oestradiol
KW - Ovary
KW - Vitamin D
UR - http://www.scopus.com/inward/record.url?scp=0027102573&partnerID=8YFLogxK
U2 - 10.1093/oxfordjournals.humrep.a137573
DO - 10.1093/oxfordjournals.humrep.a137573
M3 - Article
C2 - 1337903
AN - SCOPUS:0027102573
SN - 0268-1161
VL - 7
SP - 1357
EP - 1360
JO - Human Reproduction
JF - Human Reproduction
IS - 10
ER -