TY - JOUR
T1 - The role of aragonite matrix surface chemistry on the chondrogenic differentiation of mesenchymal stem cells
AU - Gross-Aviv, Talia
AU - Vago, Razi
N1 - Funding Information:
The author thanks Dr. L. Abramovitch-Gottlib, for her support and Ms. I. Mureinik for critically reviewing the manuscript. The work was supported in part by the USA-Israel Binational Foundation (BSF) # 2001045.
PY - 2009/2/1
Y1 - 2009/2/1
N2 - In the present research we study the effects of surface chemistry of an aragonite crystalline biomatrix on the chondrogenesis of mesenchymal stem cells (MSCs). An aragonite matrix obtained from the coral Porites lutea and a gold-coated P. lutea matrix were seeded with MSCs, with and without the addition of growth factors (GFs). Scanning electron microscopy, histochemical staining, immunofluorescence, biochemical analyses and quantitative polymerase chain reaction showed that the chemistry of the matrix influenced the differentiation process of the MSCs. The calcium carbonate composition of the coral promoted osteogenesis, while impeding cell-material contact (by gold coating) altered the differentiation lineage of MSCs towards chondrogenic fate. Supplementation of the culture medium with GFs intensified the influence of the surface composition on the differentiation of MSCs, and the synergistic effect of the biomatrix surface composition and the GFs induced chondrogenesis and facilitated maintenance of the chondrocyte phenotype. Therefore, we suggest that scaffolding material candidates for tissue engineering should be examined for their effects on the MSCs differentiation process and their effect on signal transduction events in the cells.
AB - In the present research we study the effects of surface chemistry of an aragonite crystalline biomatrix on the chondrogenesis of mesenchymal stem cells (MSCs). An aragonite matrix obtained from the coral Porites lutea and a gold-coated P. lutea matrix were seeded with MSCs, with and without the addition of growth factors (GFs). Scanning electron microscopy, histochemical staining, immunofluorescence, biochemical analyses and quantitative polymerase chain reaction showed that the chemistry of the matrix influenced the differentiation process of the MSCs. The calcium carbonate composition of the coral promoted osteogenesis, while impeding cell-material contact (by gold coating) altered the differentiation lineage of MSCs towards chondrogenic fate. Supplementation of the culture medium with GFs intensified the influence of the surface composition on the differentiation of MSCs, and the synergistic effect of the biomatrix surface composition and the GFs induced chondrogenesis and facilitated maintenance of the chondrocyte phenotype. Therefore, we suggest that scaffolding material candidates for tissue engineering should be examined for their effects on the MSCs differentiation process and their effect on signal transduction events in the cells.
KW - Aragonite biomaterial
KW - Cartilage tissue engineering
KW - Differentiation
KW - Mesenchymal stem cells (MSCs)
KW - Surface modification
UR - http://www.scopus.com/inward/record.url?scp=57549100212&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2008.10.026
DO - 10.1016/j.biomaterials.2008.10.026
M3 - Article
AN - SCOPUS:57549100212
SN - 0142-9612
VL - 30
SP - 770
EP - 779
JO - Biomaterials
JF - Biomaterials
IS - 5
ER -