The role of Hsp90 in cell response to hyperthermia

V. Yavelsky, O. Vais, B. Piura, M. Wolfson, A. Rabinovich, V. Fraifeld

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

(1) Preincubation of SKOV3 human ovarian carcinoma cells with a non-toxic dose of Geldanamycin resulted in exacerbation of hyperthermia-induced cytotoxicity and re-distribution of dying cells toward necrosis. (2) Exposure of primary human ovarian carcinoma cells to mild hyperthermia (42°C for 2 h) led, after a recovery period of 16 h, to several-fold increase in the levels of Hsp90 and ErbB2, to a moderate decrease in the levels of phospho-Erk1/2, whereas the level of β-catenin appeared to be unchanged. (3) The inhibitors of Hsp90 (Geldanamycin and Novobiocin) significantly affected the cell signaling of heat-pretreated cultures. (4) The results suggest that Hsp90 plays a pivotal role in cell response to hyperthermia. (5) A combination of Hsp90 inhibitors with hyperthermia may considerably increase the efficacy of thermotherapy.

Original languageEnglish
Pages (from-to)509-514
Number of pages6
JournalJournal of Thermal Biology
Volume29
Issue number7-8 SPEC. ISS.
DOIs
StatePublished - 1 Oct 2004
Externally publishedYes

Keywords

  • Apoptosis/Necrosis
  • Cell signaling
  • Heat shock protein 90 (Hsp90)
  • Human SKOV3 and primary ovarian carcinoma cells
  • Hyperthermia
  • Inhibitors of Hsp90

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • General Agricultural and Biological Sciences
  • Developmental Biology

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