The role of Hsp90 in cell response to hyperthermia

V. Yavelsky; O. Vais; B. Piura; M. Wolfson; A. Rabinovich; V. Fraifeld

doi:10.1016/j.jtherbio.2004.08.078

The role of Hsp90 in cell response to hyperthermia

V. Yavelsky, O. Vais, B. Piura, M. Wolfson, A. Rabinovich, V. Fraifeld

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

(1) Preincubation of SKOV3 human ovarian carcinoma cells with a non-toxic dose of Geldanamycin resulted in exacerbation of hyperthermia-induced cytotoxicity and re-distribution of dying cells toward necrosis. (2) Exposure of primary human ovarian carcinoma cells to mild hyperthermia (42°C for 2 h) led, after a recovery period of 16 h, to several-fold increase in the levels of Hsp90 and ErbB2, to a moderate decrease in the levels of phospho-Erk1/2, whereas the level of β-catenin appeared to be unchanged. (3) The inhibitors of Hsp90 (Geldanamycin and Novobiocin) significantly affected the cell signaling of heat-pretreated cultures. (4) The results suggest that Hsp90 plays a pivotal role in cell response to hyperthermia. (5) A combination of Hsp90 inhibitors with hyperthermia may considerably increase the efficacy of thermotherapy.

Original language	English
Pages (from-to)	509-514
Number of pages	6
Journal	Journal of Thermal Biology
Volume	29
Issue number	7-8 SPEC. ISS.
DOIs	https://doi.org/10.1016/j.jtherbio.2004.08.078
State	Published - 1 Oct 2004

Keywords

Apoptosis/Necrosis
Cell signaling
Heat shock protein 90 (Hsp90)
Human SKOV3 and primary ovarian carcinoma cells
Hyperthermia
Inhibitors of Hsp90

ASJC Scopus subject areas

Biochemistry
Physiology
General Agricultural and Biological Sciences
Developmental Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jtherbio.2004.08.078

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title = "The role of Hsp90 in cell response to hyperthermia",

abstract = "(1) Preincubation of SKOV3 human ovarian carcinoma cells with a non-toxic dose of Geldanamycin resulted in exacerbation of hyperthermia-induced cytotoxicity and re-distribution of dying cells toward necrosis. (2) Exposure of primary human ovarian carcinoma cells to mild hyperthermia (42°C for 2 h) led, after a recovery period of 16 h, to several-fold increase in the levels of Hsp90 and ErbB2, to a moderate decrease in the levels of phospho-Erk1/2, whereas the level of β-catenin appeared to be unchanged. (3) The inhibitors of Hsp90 (Geldanamycin and Novobiocin) significantly affected the cell signaling of heat-pretreated cultures. (4) The results suggest that Hsp90 plays a pivotal role in cell response to hyperthermia. (5) A combination of Hsp90 inhibitors with hyperthermia may considerably increase the efficacy of thermotherapy.",

keywords = "Apoptosis/Necrosis, Cell signaling, Heat shock protein 90 (Hsp90), Human SKOV3 and primary ovarian carcinoma cells, Hyperthermia, Inhibitors of Hsp90",

author = "V. Yavelsky and O. Vais and B. Piura and M. Wolfson and A. Rabinovich and V. Fraifeld",

year = "2004",

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doi = "10.1016/j.jtherbio.2004.08.078",

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AU - Yavelsky, V.

AU - Vais, O.

AU - Piura, B.

AU - Wolfson, M.

AU - Rabinovich, A.

AU - Fraifeld, V.

PY - 2004/10/1

Y1 - 2004/10/1

N2 - (1) Preincubation of SKOV3 human ovarian carcinoma cells with a non-toxic dose of Geldanamycin resulted in exacerbation of hyperthermia-induced cytotoxicity and re-distribution of dying cells toward necrosis. (2) Exposure of primary human ovarian carcinoma cells to mild hyperthermia (42°C for 2 h) led, after a recovery period of 16 h, to several-fold increase in the levels of Hsp90 and ErbB2, to a moderate decrease in the levels of phospho-Erk1/2, whereas the level of β-catenin appeared to be unchanged. (3) The inhibitors of Hsp90 (Geldanamycin and Novobiocin) significantly affected the cell signaling of heat-pretreated cultures. (4) The results suggest that Hsp90 plays a pivotal role in cell response to hyperthermia. (5) A combination of Hsp90 inhibitors with hyperthermia may considerably increase the efficacy of thermotherapy.

AB - (1) Preincubation of SKOV3 human ovarian carcinoma cells with a non-toxic dose of Geldanamycin resulted in exacerbation of hyperthermia-induced cytotoxicity and re-distribution of dying cells toward necrosis. (2) Exposure of primary human ovarian carcinoma cells to mild hyperthermia (42°C for 2 h) led, after a recovery period of 16 h, to several-fold increase in the levels of Hsp90 and ErbB2, to a moderate decrease in the levels of phospho-Erk1/2, whereas the level of β-catenin appeared to be unchanged. (3) The inhibitors of Hsp90 (Geldanamycin and Novobiocin) significantly affected the cell signaling of heat-pretreated cultures. (4) The results suggest that Hsp90 plays a pivotal role in cell response to hyperthermia. (5) A combination of Hsp90 inhibitors with hyperthermia may considerably increase the efficacy of thermotherapy.

KW - Apoptosis/Necrosis

KW - Cell signaling

KW - Heat shock protein 90 (Hsp90)

KW - Human SKOV3 and primary ovarian carcinoma cells

KW - Hyperthermia

KW - Inhibitors of Hsp90

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U2 - 10.1016/j.jtherbio.2004.08.078

DO - 10.1016/j.jtherbio.2004.08.078

M3 - Article

AN - SCOPUS:4744359868

SN - 0306-4565

VL - 29

SP - 509

EP - 514

JO - Journal of Thermal Biology

JF - Journal of Thermal Biology

IS - 7-8 SPEC. ISS.

ER -

The role of Hsp90 in cell response to hyperthermia

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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