The role of protein kinase a in membrane fluidization induced by acetylcholine

E. Alfahe, A. H. Parola, Z. Priel

Research output: Contribution to journalArticlepeer-review

Abstract

The cilia are projections of the cell surface and are covered with membrane which are continuous with the cell surface membrane The effect of acetylcholine (Ach) on mucus transporting cilia in a freshly excised frog palate was examined by Fluorescence Polarization (P) method using the non-penetrating lipophilic membrane probet-(4-tnmethylammonium phenyl)-6-phenyl-l,3,5-hexatriene, p-to!uenesulfonate (TMA-DPH) We found that Ach (1-100 mM) lowered P (maximum 11% at 10 mM) The effect of Ach was abolished after treatment with the following reagents ( 1 ) Atropine (100 mM), a well known muscarinic inhibitor, (2) Thapsigargin (1 mM). which inhibits microsomal Ca+2-ATPase, (3) Quinidine ( I mM), apamin (0 5 mM), cesium (5 mM) and charybdotoxin (20 nM), blockers of potassium-calcium activated channels, and (4) H-89 (5 mM), an inhibitor of protein kinase A (PK.A) Cytochalasin B (18 mM) or colchicine (18 mM), well known cytoskeleton reagents, reduced the effect of Ach, while the addition of both cytoskeleton reagents, each at 18 mM, completely abolished the Ach effect observed above We conclude that intracellular free Ca+2, the cytoskeleton and PKA are involved in the membrane fluidization induced by Ach.

Original languageEnglish
Pages (from-to)A1057
JournalFASEB Journal
Volume11
Issue number9
StatePublished - 1 Dec 1997

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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