TY - JOUR
T1 - The role of pyruvate hub enzymes in supplying carbon precursors for fatty acid synthesis in photosynthetic microalgae
AU - Shtaida, Nastassia
AU - Khozin-Goldberg, Inna
AU - Boussiba, Sammy
N1 - Funding Information:
The authors acknowledge the financial support by the European Commission’s Seventh Framework Program for Research and Technology Development (FP7), project GIAVAP, Grant No. 266401. NS acknowledges support from the Albert Katz International School for Desert Studies and the Kreitman School of Advanced Graduate Studies at Ben-Gurion University.
Publisher Copyright:
© 2015 Springer Science+Business Media Dordrecht.
PY - 2015/9/17
Y1 - 2015/9/17
N2 - Photosynthetic microalgae are currently the focus of basic and applied research due to an ever-growing interest in renewable energy resources. This review discusses the role of carbon-unit supply for the production of acetyl-CoA, a direct precursor of fatty acid biosynthesis and the primary building block of the growing acyl chains for the purpose of triacylglycerol (TAG) production in photosynthetic microalgae under stressful conditions. It underscores the importance of intraplastidic acetyl-CoA generation for storage lipid accumulation. The main focus is placed on two enzymatic steps linking the central carbon metabolism and fatty acid synthesis, namely the reactions catalyzed by the plastidic isoform of pyruvate kinase and the chloroplastic pyruvate dehydrogenase complex. Alternative routes for plastidic acetyl-CoA synthesis are also reviewed. A separate section is devoted to recent advances in functional genomics studies related to fatty acid and TAG biosynthesis.
AB - Photosynthetic microalgae are currently the focus of basic and applied research due to an ever-growing interest in renewable energy resources. This review discusses the role of carbon-unit supply for the production of acetyl-CoA, a direct precursor of fatty acid biosynthesis and the primary building block of the growing acyl chains for the purpose of triacylglycerol (TAG) production in photosynthetic microalgae under stressful conditions. It underscores the importance of intraplastidic acetyl-CoA generation for storage lipid accumulation. The main focus is placed on two enzymatic steps linking the central carbon metabolism and fatty acid synthesis, namely the reactions catalyzed by the plastidic isoform of pyruvate kinase and the chloroplastic pyruvate dehydrogenase complex. Alternative routes for plastidic acetyl-CoA synthesis are also reviewed. A separate section is devoted to recent advances in functional genomics studies related to fatty acid and TAG biosynthesis.
KW - Chloroplast pyruvate dehydrogenase complex
KW - Chloroplast pyruvate kinase
KW - Lipid biosynthesis
KW - Microalgae
UR - http://www.scopus.com/inward/record.url?scp=84939572568&partnerID=8YFLogxK
U2 - 10.1007/s11120-015-0136-7
DO - 10.1007/s11120-015-0136-7
M3 - Review article
C2 - 25846135
AN - SCOPUS:84939572568
SN - 0166-8595
VL - 125
SP - 407
EP - 422
JO - Photosynthesis Research
JF - Photosynthesis Research
IS - 3
ER -
